Tan, JonathanWatanabe, Takeshi2021-09-212021-09-212045-2322http://hdl.handle.net/1885/248286Development of lymphoid tissue is determined by interactions between stromal lymphoid tissue organiser (LTo) and hematopoietic lymphoid tissue inducer (LTi) cells. A failure for LTo to receive appropriate activating signals during embryogenesis through lymphotoxin engagement leads to a complete cessation of lymph node (LN) and Peyer’s patch development, identifying LTo as a key stromal population for lymphoid tissue organogenesis. However, little is known about the equivalent stromal cells that induce spleen development. Here, by dissociating neonatal murine spleen stromal tissue for re-aggregation and transplant into adult mouse recipients, we have identified a MAdCAM-1+CD31+CD201+ spleen stromal organizer cell-type critical for new tissue formation. This finding provides an insight into the regulation of post-natal spleen tissue organogenesis, and could be exploited in the development of spleen regenerative therapiesThis work was supported by MEXT, Japan (Grant-in-Aid for Scientific Research on Priority Areas 19059015 to T.W.) and NHMRC, Australia (CJ Martin Biomedical Postdoctoral Fellowship 1013553 to J.K.H.T).application/pdfen-AU© The Author(s) 2017https://creativecommons.org/licenses/by/4.0/201710.1038/srep404012020-11-23Creative Commons License (Attribution 4.0 International)