Molecular evolution of VH9 germline genes isolated from DBA, BALB, 129 and C57BL mouse strains and sublines

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dc.contributor.authorZylstra, Paula
dc.contributor.authorFranklin, Andrew
dc.contributor.authorHassan, Karl
dc.contributor.authorPowell, Kim
dc.contributor.authorSteele, Edward J
dc.contributor.authorBlanden, Robert
dc.date.accessioned2015-12-13T22:17:41Z
dc.date.available2015-12-13T22:17:41Z
dc.date.issued2003
dc.date.updated2015-12-11T07:35:51Z
dc.description.abstractWe have used the polymerase chain reaction (PCR) in an attempt to clone and sequence the exons and hitherto unavailable contiguous flanks of all members of the small VH9 germline gene family from inbred mouse strains and sublines that have had a common ancestry within the last century, and to analyze the molecular evolution of these sequences. Fifteen genuine germline genes were isolated (designated VH9.1 through VH9.15) from strains and sublines of DBA, BALB, 129 and C57BL inbred mice. Of the 15 genuine isolates, nine are novel: seven sequences from DBA strains and sublines (VH9.3 to VH9.9) and two sequences from C57BL strains (VH9.13 and VH9.14). We have identified sequencing errors and PCR recombinant artefacts in previously published sequences. We detected no sequence divergence of individual genes shared by the strains and sublines studied. However, we isolated two genes from DBA strains and sublines, VH9.1 and VH9.3, that differ only by five nucleotides encoding three amino acid changes that are concentrated within a 33 nucleotide (11 codon) region. Of these 11 codons, eight encode a putative antigen binding site. There were no differences in the remaining 733 nucleotides sequenced (including both 5′ and 3′ flanking regions). Potential explanations for the generation of VH9.1 and VH9.3 are discussed.
dc.identifier.issn0093-7711
dc.identifier.urihttp://hdl.handle.net/1885/71268
dc.publisherSpringer
dc.sourceImmunogenetics
dc.subjectKeywords: amino acid; immunoglobulin; nucleotide; animal experiment; article; exon; gene isolation; gene sequence; germ line; molecular cloning; molecular evolution; mouse; multigene family; nonhuman; nucleotide sequence; polymerase chain reaction; priority journal Evolution; Germline; Immunoglobulin; Mouse; V gene
dc.titleMolecular evolution of VH9 germline genes isolated from DBA, BALB, 129 and C57BL mouse strains and sublines
dc.typeJournal article
local.bibliographicCitation.issue3
local.bibliographicCitation.lastpage188
local.bibliographicCitation.startpage182
local.contributor.affiliationZylstra, Paula, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationFranklin, Andrew, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationHassan, Karl, University of Wollongong
local.contributor.affiliationPowell, Kim, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationSteele, Edward J, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationBlanden, Robert, College of Medicine, Biology and Environment, ANU
local.contributor.authoremailrepository.admin@anu.edu.au
local.contributor.authoruidZylstra, Paula, u3888118
local.contributor.authoruidFranklin, Andrew, u9913738
local.contributor.authoruidPowell, Kim, u850910
local.contributor.authoruidSteele, Edward J, a145554
local.contributor.authoruidBlanden, Robert, u7100504
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.absfor110706 - Immunogenetics (incl. Genetic Immunology)
local.identifier.ariespublicationMigratedxPub2635
local.identifier.citationvolume55
local.identifier.doi10.1007/s00251-003-0565-x
local.identifier.scopusID2-s2.0-0038007123
local.identifier.uidSubmittedByMigrated
local.type.statusPublished Version

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