Effects of bacterial products on enterocyte-macrophage interactions in vitro
dc.contributor.author | Tyrer, Peter | |
dc.contributor.author | Bean, Elaine | |
dc.contributor.author | Foxwell, A.R | |
dc.contributor.author | Pavli, Paul | |
dc.date.accessioned | 2015-12-08T22:21:48Z | |
dc.date.issued | 2011 | |
dc.date.updated | 2016-02-24T11:32:17Z | |
dc.description.abstract | We describe a coculture model of a human intestinal epithelial cell line and human peripheral blood monocytes in which monocytes differentiate into cells with features of resident intestinal macrophages. Caco-2 cells are grown on the lower surface of a semipermeable filter with pore size of 3μm (Transwells®) until they differentiate into enterocytes. Peripheral-blood monocytes are added and the co-culture incubated for two days. Monocytes migrate through the pores of the membrane, come into direct contact with the basolateral surfaces of the epithelial cell monolayer, and develop characteristics of resident intestinal macrophages including downregulation of CD14 expression and reduced pro-inflammatory cytokine responses (IL-8, TNF and IL-1β) to bacterial products. The apical application of lipopolysaccharide (LPS) and muramyl dipeptide (MDP) resulted in an increased number of integrated monocytes, but abrogated the downregulation of CD14 expression and the diminished cytokine responses. MDP also reduced tight-junctional integrity, whilst LPS had no effect. These data indicate that LPS and MDP have significant pathophysiological effects on enterocyte-monocyte interactions, and confirm other studies that demonstrate that enterocytes and their products influence monocyte differentiation. This model may be useful in providing insights into the interaction between monocytes, epithelial cells and intestinal bacteria in health and disease. | |
dc.identifier.issn | 0006-291X | |
dc.identifier.uri | http://hdl.handle.net/1885/32282 | |
dc.publisher | Academic Press | |
dc.source | Biochemical and Biophysical Research Communications | |
dc.subject | Keywords: CD14 antigen; interleukin 1beta; interleukin 8; lipopolysaccharide; muramyl dipeptide; tumor necrosis factor alpha; adenocarcinoma; antigen expression; article; cell differentiation; cell interaction; coculture; controlled study; cytokine response; down r Enterocytes; Intestine; Macrophages; Monocytes; Mucosal immunology; Pathogen-associated molecular pattern molecules | |
dc.title | Effects of bacterial products on enterocyte-macrophage interactions in vitro | |
dc.type | Journal article | |
local.bibliographicCitation.issue | 2 | |
local.bibliographicCitation.lastpage | 341 | |
local.bibliographicCitation.startpage | 336 | |
local.contributor.affiliation | Tyrer, Peter, Canberra Hospital | |
local.contributor.affiliation | Bean, Elaine, Canberra Hospital | |
local.contributor.affiliation | Foxwell, A.R, University of Canberra | |
local.contributor.affiliation | Pavli, Paul, College of Medicine, Biology and Environment, ANU | |
local.contributor.authoremail | u3683784@anu.edu.au | |
local.contributor.authoruid | Pavli, Paul, u3683784 | |
local.description.embargo | 2037-12-31 | |
local.description.notes | Imported from ARIES | |
local.identifier.absfor | 110307 - Gastroenterology and Hepatology | |
local.identifier.ariespublication | u4971216xPUB90 | |
local.identifier.citationvolume | 413 | |
local.identifier.doi | 10.1016/j.bbrc.2011.08.100 | |
local.identifier.scopusID | 2-s2.0-80053093379 | |
local.identifier.thomsonID | 000295443400032 | |
local.identifier.uidSubmittedBy | u4971216 | |
local.type.status | Published Version |