Effects of bacterial products on enterocyte-macrophage interactions in vitro

dc.contributor.authorTyrer, Peter
dc.contributor.authorBean, Elaine
dc.contributor.authorFoxwell, A.R
dc.contributor.authorPavli, Paul
dc.date.accessioned2015-12-08T22:21:48Z
dc.date.issued2011
dc.date.updated2016-02-24T11:32:17Z
dc.description.abstractWe describe a coculture model of a human intestinal epithelial cell line and human peripheral blood monocytes in which monocytes differentiate into cells with features of resident intestinal macrophages. Caco-2 cells are grown on the lower surface of a semipermeable filter with pore size of 3μm (Transwells®) until they differentiate into enterocytes. Peripheral-blood monocytes are added and the co-culture incubated for two days. Monocytes migrate through the pores of the membrane, come into direct contact with the basolateral surfaces of the epithelial cell monolayer, and develop characteristics of resident intestinal macrophages including downregulation of CD14 expression and reduced pro-inflammatory cytokine responses (IL-8, TNF and IL-1β) to bacterial products. The apical application of lipopolysaccharide (LPS) and muramyl dipeptide (MDP) resulted in an increased number of integrated monocytes, but abrogated the downregulation of CD14 expression and the diminished cytokine responses. MDP also reduced tight-junctional integrity, whilst LPS had no effect. These data indicate that LPS and MDP have significant pathophysiological effects on enterocyte-monocyte interactions, and confirm other studies that demonstrate that enterocytes and their products influence monocyte differentiation. This model may be useful in providing insights into the interaction between monocytes, epithelial cells and intestinal bacteria in health and disease.
dc.identifier.issn0006-291X
dc.identifier.urihttp://hdl.handle.net/1885/32282
dc.publisherAcademic Press
dc.sourceBiochemical and Biophysical Research Communications
dc.subjectKeywords: CD14 antigen; interleukin 1beta; interleukin 8; lipopolysaccharide; muramyl dipeptide; tumor necrosis factor alpha; adenocarcinoma; antigen expression; article; cell differentiation; cell interaction; coculture; controlled study; cytokine response; down r Enterocytes; Intestine; Macrophages; Monocytes; Mucosal immunology; Pathogen-associated molecular pattern molecules
dc.titleEffects of bacterial products on enterocyte-macrophage interactions in vitro
dc.typeJournal article
local.bibliographicCitation.issue2
local.bibliographicCitation.lastpage341
local.bibliographicCitation.startpage336
local.contributor.affiliationTyrer, Peter, Canberra Hospital
local.contributor.affiliationBean, Elaine, Canberra Hospital
local.contributor.affiliationFoxwell, A.R, University of Canberra
local.contributor.affiliationPavli, Paul, College of Medicine, Biology and Environment, ANU
local.contributor.authoremailu3683784@anu.edu.au
local.contributor.authoruidPavli, Paul, u3683784
local.description.embargo2037-12-31
local.description.notesImported from ARIES
local.identifier.absfor110307 - Gastroenterology and Hepatology
local.identifier.ariespublicationu4971216xPUB90
local.identifier.citationvolume413
local.identifier.doi10.1016/j.bbrc.2011.08.100
local.identifier.scopusID2-s2.0-80053093379
local.identifier.thomsonID000295443400032
local.identifier.uidSubmittedByu4971216
local.type.statusPublished Version

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