A novel binding assay to assess specificity of monoclonal antibodies

Date

Authors

Warren, Hilary

Bettadapura, Jayaram

Publisher

Elsevier

Abstract

Dynabeads® TALON™ are uniform superparamagnetic polystyrene beads of 1μm diameter that bind 6-His-tagged recombinant proteins through Co++-affinity binding, and are normally used for protein purification. We have used these beads to bind 6-His-rNKp30

Keywords

Keywords: cobalt; monoclonal antibody; polystyrene; recombinant protein; antibody specificity; antigen binding; binding affinity; cell differentiation; conference paper; controlled study; human; human cell; leukocyte; priority journal; protein purification; Antibod 6-His recombinant protein; Magnetic beads; Monoclonal antibody; NK cells; NKp30; NKp46

URI

http://hdl.handle.net/1885/81218

Collections

ANU Research Publications

Source

Journal of Immunological Methods

Type

Journal article

DOI

10.1016/j.jim.2005.07.011

Restricted until

2037-12-31

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Description

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