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Monitoring lymphocyte proliferation in vitro and in vivo with the intracellular fluorescent dye carboxyfluorescein diacetate succinimidyl ester

Date

Authors

Quah, Ben

Warren, Hilary

Parish, Christopher

Publisher

Nature Publishing Group

Abstract

This protocol outlines the carboxyfluorescein diacetate succinimidyl ester (CFSE) method for following the proliferation of human lymphocytes in vitro and mouse lymphocytes both in vitro and in vivo. The method relies on the ability of CFSE to covalently

Keywords

Keywords: 5 (6) carboxyfluorescein diacetate succinimidyl ester; 5-(6)-carboxyfluorescein diacetate succinimidyl ester; fluorescein derivative; fluorescent dye; succinimide derivative; unclassified drug; carboxyfluorescein diacetate succinimidyl ester; cell protein

URI

http://hdl.handle.net/1885/25647

Collections

ANU Research Publications

Source

Nature Protocols

Type

Journal article

DOI

10.1038/nprot.2007.296

Restricted until

2037-12-31

Downloads

File

Description

01_Quah_Monitoring_lymphocyte_2007.pdf (484.76 KB)