Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles

Date

2011-03-02

Authors

Arita, Yoshihiko
Torres-Mapa, Maria Leilani
Lee, Woei Ming
Čižmár, Tomáš
Campbell, Paul
Gunn-Moore, Frank J.
Dholakia, Kishan

Journal Title

Journal ISSN

Volume Title

Publisher

American Institute of Physics (AIP)

Abstract

We demonstrate laser-induced breakdown of an optically trapped nanoparticle with a nanosecond laser pulse. Controllable cavitation within a microscope sample was achieved, generating shear stress to monolayers of live cells. This efficiently permeabilize their plasma membranes. We show that this technique is an excellent tool for plasmid-DNA transfection of cells with both reduced energy requirements and reduced cell lysis compared to previously reported approaches. Simultaneous multisite targeted nanosurgery of cells is also demonstrated using a spatial light modulator for parallelizing the technique.

Description

Keywords

Keywords: Cell lysis; DNA transfection; Gene transfection; Laser induced breakdown; Live cell; Multi-site; Nanosecond laser pulse; Nanosurgery; Parallelizing; Plasma membranes; Reduced energy; Spatial light modulators; Trapped nanoparticles; Cell membranes; Genes;

Citation

Source

Applied Physics Letters

Type

Journal article

Book Title

Entity type

Access Statement

License Rights

DOI

10.1063/1.3554415

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