Multinucleation and preservation of nuclear integrity of macrophages

Date

1999

Authors

Sorimachi, Kenji
Naora, Hiroto
Akimoto, Kazumi
Niwa, Akira

Journal Title

Journal ISSN

Volume Title

Publisher

Academic Press

Abstract

Rat bone marrow-derived macrophages formed multinucleated giant cells spontaneously when cultured in slide glass chambers or when induced with the polyanion acetyl lignin. Nuclei in such cells tended to cluster in distinct rings. DNA fragmentation appeared to occur in multinucleated cells, as detected by 3' end-labeling. Southern blot analyses, using probes specific for nucleolar and non-nucleolar genes, indicated that chromatin DNA was fragmented whereas nucleolar DNA was relatively intact. Autoradiography revealed preservation, in multinucleated cells, of nucleoli into which radiolabeled uridine was incorporated. Multinucleated macrophages appeared to eventually fragment. Preserved integrity of nucleoli seems to be a feature of macrophage multinucleation, a process which apparently culminates in cell death.

Description

Keywords

Keywords: acetic acid derivative; DNA fragment; glass; lignin; polyanion; uridine; animal cell; article; autoradiography; bone marrow; cell culture; chromatin; controlled study; giant cell; macrophage; multinuclear cell; nonhuman; nucleolus; rat; southern blotting; Chromatin DNA; Macrophages; Multinucleation; Nucleolar DNA

Citation

Source

Cell Biology International

Type

Journal article

Book Title

Entity type

Access Statement

License Rights

Restricted until

2037-12-31