Multinucleation and preservation of nuclear integrity of macrophages
Date
1999
Authors
Sorimachi, Kenji
Naora, Hiroto
Akimoto, Kazumi
Niwa, Akira
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Volume Title
Publisher
Academic Press
Abstract
Rat bone marrow-derived macrophages formed multinucleated giant cells spontaneously when cultured in slide glass chambers or when induced with the polyanion acetyl lignin. Nuclei in such cells tended to cluster in distinct rings. DNA fragmentation appeared to occur in multinucleated cells, as detected by 3' end-labeling. Southern blot analyses, using probes specific for nucleolar and non-nucleolar genes, indicated that chromatin DNA was fragmented whereas nucleolar DNA was relatively intact. Autoradiography revealed preservation, in multinucleated cells, of nucleoli into which radiolabeled uridine was incorporated. Multinucleated macrophages appeared to eventually fragment. Preserved integrity of nucleoli seems to be a feature of macrophage multinucleation, a process which apparently culminates in cell death.
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Keywords
Keywords: acetic acid derivative; DNA fragment; glass; lignin; polyanion; uridine; animal cell; article; autoradiography; bone marrow; cell culture; chromatin; controlled study; giant cell; macrophage; multinuclear cell; nonhuman; nucleolus; rat; southern blotting; Chromatin DNA; Macrophages; Multinucleation; Nucleolar DNA
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Source
Cell Biology International
Type
Journal article
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2037-12-31
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