Incorporation of chlorinated analogues of aliphatic amino acids during cell-free protein synthesis

Date

2011

Authors

Stigers, Dannon
Watts, Zachary
Hennessy, James E
Kim, Hye-Kyung
Martini, Romeo
Taylor, Matthew C
Ozawa, Kiyoshi
Keillor, Jeffrey W
Dixon, Nicholas Edward
Easton, Christopher

Journal Title

Journal ISSN

Volume Title

Publisher

Royal Society of Chemistry

Abstract

3-Chloro-Abu and 4-chloro-Nva are biosynthetically incorporated into E. coli peptidyl-Pro cis-trans isomerase B, as substitutes for Val and Leu, respectively. The extent of incorporation is up to ∼90%, and substituted protein is catalytically active. By

Description

Keywords

Keywords: amino acid derivative; chlorine derivative; cis trans isomerase; cis trans isomerase b; isoleucine; leucine; unclassified drug; valine; amino acid substitution; article; cell free protein synthesis; chlorination; controlled study; Escherichia coli; nonhum

Citation

Collections

Source

Chemical Communications

Type

Journal article

Book Title

Entity type

Access Statement

License Rights

DOI

10.1039/C0CC02879G

Restricted until

2037-12-31