Delivery of a multivalent scrambled antigen vaccine induces broad spectrum immunity and protection against tuberculosis

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dc.contributor.authorWest, Nicholas P
dc.contributor.authorThomson, Scott
dc.contributor.authorTriccas, James A
dc.contributor.authorMedveczky, C
dc.contributor.authorRamshaw, Ian
dc.contributor.authorBritton, Warwick
dc.date.accessioned2015-12-10T22:20:04Z
dc.date.issued2011
dc.date.updated2016-02-24T08:56:41Z
dc.description.abstractThe development of effective anti-Tuberculosis (TB) vaccines is an important step towards improved control of TB in high burden countries. Subunit vaccines are advantageous in terms of safety, particularly in the context of high rates of HIV co-infection, but they must contain sufficient Mycobacterium tuberculosis antigens to stimulate immunity in genetically diverse human populations. We have used a novel approach to develop a synthetic scrambled antigen vaccine (TB-SAVINE), comprised of overlapping, recombined peptides from four M. tuberculosis proteins, Ag85B, ESAT-6, PstS3 and Mpt83, each of which is immunogenic and protective against experimental TB. This polyvalent TB-SAVINE construct stimulated CD4 and CD8T cell responses against the individual proteins and M. tuberculosis in C57BL/6 and Balb/c mice, when delivered as DNA, Fowl Pox Virus or Vaccinia Virus vaccines. In addition, the DNA-TBS vaccine induced protective immunity against pulmonary M. tuberculosis infection in C57BL/6 mice. Co-immunization of Balb/c mice with virally expressed TBS and HIV1-SAVINE vaccine stimulated strong T cell responses to both the M. tuberculosis and HIV proteins, indicating no effects of antigenic competition. Further development of this TB-SAVINE vaccine expressing components from multiple M. tuberculosis proteins may prove an effective vaccine candidate against TB, which could potentially form part of a safe, combined preventative strategy together with HIV immunisations.
dc.identifier.issn0264-410X
dc.identifier.urihttp://hdl.handle.net/1885/51778
dc.publisherElsevier
dc.sourceVaccine
dc.subjectKeywords: bacterial protein; CD4 antigen; DNA vaccine; Human immunodeficiency virus protein; Human immunodeficiency virus vaccine; vaccinia vaccine; article; bacterial immunity; CD8+ T lymphocyte; controlled study; female; Fowlpox virus; Human immunodeficiency viru Coimmunization; HIV; Savine; Tuberculosis; Vaccine
dc.titleDelivery of a multivalent scrambled antigen vaccine induces broad spectrum immunity and protection against tuberculosis
dc.typeJournal article
local.bibliographicCitation.issue44
local.bibliographicCitation.lastpage7765
local.bibliographicCitation.startpage7759
local.contributor.affiliationWest, Nicholas P, Centenary Institute of Cancer Medicine and Cell Biology
local.contributor.affiliationThomson, Scott, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationTriccas, James A, University of Sydney
local.contributor.affiliationMedveczky, C, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationRamshaw, Ian, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationBritton, Warwick, University of Sydney
local.contributor.authoremailrepository.admin@anu.edu.au
local.contributor.authoruidThomson, Scott, u9711363
local.contributor.authoruidMedveczky, C, u8104438
local.contributor.authoruidRamshaw, Ian, u8202754
local.description.embargo2037-12-31
local.description.notesImported from ARIES
local.identifier.absfor110704 - Cellular Immunology
local.identifier.ariespublicationf5625xPUB232
local.identifier.citationvolume29
local.identifier.doi10.1016/j.vaccine.2011.07.109
local.identifier.scopusID2-s2.0-80053587106
local.identifier.thomsonID000296546900031
local.identifier.uidSubmittedByf5625
local.type.statusPublished Version

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