Translational incorporation of modified phenylalanines and tyrosines during cell-free protein synthesis

Date

2020

Authors

Wang, Zhongqiang
Matthews, Hayden

Journal Title

Journal ISSN

Volume Title

Publisher

Royal Society of Chemistry

Abstract

Inherent promiscuity of bacterial translation is demonstrated by mass spectrometric quantification of the translational incorporation of ring-substituted phenylalanines and tyrosines bearing fluoro-, hydroxyl-, methyl-, chloro- and nitro-groups in an E. coli-derived cell-free system. Competitive studies using the cell-free system show that the aminoacyl-tRNA synthetases (aaRS) have at least two orders of magnitude higher specificity for the native substrate over these structural analogues, which correlates with studies on the purified synthetase.

Description

Keywords

Citation

Source

RSC Advances

Type

Journal article

Book Title

Entity type

Access Statement

Open Access

License Rights

Creative Commons Attribution licence

DOI

10.1039/d0ra00655f

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