The merozoite-specific protein, TgGRA11B, identified as a component of the Toxoplasma gondii parasitophorous vacuole in a tachyzoite expression model

Date

2017-05-17

Authors

Ramakrishnan, Chandra
Walker, Robert A.
Eichenberger, Ramon M.
Hehl, Adrian B.
Smith, Nicholas

Journal Title

Journal ISSN

Volume Title

Publisher

Elsevier

Abstract

The apicomplexan, Toxoplasma gondii, infects all warm-blooded animals as intermediate hosts but only felids as definitive hosts. Dense granule proteins are critical for the survival of Toxoplasma within host cells but, whilst these proteins have been studied intensively in tachyzoites, little is known about their expression in the coccidian stages in the cat intestine. Transcriptomic profiling indicates that two putative dense granule proteins, TgGRA11A and TgGRA11B, are expressed uniquely in merozoites. Immunofluorescent microscopy of Toxoplasma-infected cat intestine and tachyzoites engineered to express TgGRA11B, reveals that it is a dense granule protein that traffics into the parasitophorous vacuole and its membrane.

Description

Keywords

Toxoplasma gondii, Coccidia, Merozoite, TgGRA11B, Dense granule, Parasitophorous vacuole

Citation

Source

International Journal for Parasitology

Type

Journal article

Book Title

Entity type

Access Statement

License Rights

DOI

10.1016/j.ijpara.2017.04.001

Restricted until

2037-12-31