Galactose-functionalised PCL nanofibre scaffolds to attenuate inflammatory action of astrocytes in vitro and in vivo

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dc.contributor.authorMaclean, Francesca
dc.contributor.authorLau, Chew L
dc.contributor.authorOzergun, Sam
dc.contributor.authorO'Shea, Ross D.
dc.contributor.authorCederfur, Cecilia
dc.contributor.authorWang, J
dc.contributor.authorHealy, Kevin E
dc.contributor.authorWalker, Frederick R.
dc.contributor.authorTomas, Doris
dc.contributor.authorHorne, Malcolm K.
dc.contributor.authorBeart, Philip M
dc.contributor.authorNisbet, David
dc.date.accessioned2020-12-20T20:56:47Z
dc.date.available2020-12-20T20:56:47Z
dc.date.issued2017
dc.date.updated2020-11-23T10:34:48Z
dc.description.abstractAstrocytes represent an attractive therapeutic target for the treatment of traumatic brain injury in the glial scar, which inhibits functional repair and recovery if persistent. Many biomaterial systems have been investigated for neural tissue engineering applications, including electrospun nanofibres, which are a favourable biomaterial as they can mimic the fibrous architecture of the extracellular matrix, and are conveniently modified to present biologically relevant cues to aid in regeneration. Here, we synthesised a novel galactose-presenting polymer, poly(L-lysine)–lactobionic acid (PLL–LBA), for use in layer-by-layer (LbL) functionalisation of poly(ε-caprolactone) (PCL) nanofibres, to covalently attach galactose moieties to the nanofibre scaffold surface. We have assessed the use of this novel biomaterial system in vitro and in vivo, and have shown, for the first time, the ability of galactose to maintain an attenuated inflammatory profile of astrocytes in culture, and to increase the survival of neurons after traumatic injury, as compared to control PCL nanofibres. This study highlights the importance of galactose in controlling the astrocytic response, and provides a promising biomaterial system to deliver the essential morphological and biological cues to achieve functional repair after traumatic brain injury.
dc.format.mimetypeapplication/pdfen_AU
dc.identifier.issn2050-750X
dc.identifier.urihttp://hdl.handle.net/1885/218059
dc.language.isoen_AUen_AU
dc.publisherRoyal Society of Chemistry
dc.sourceJournal of Materials Chemistry B
dc.titleGalactose-functionalised PCL nanofibre scaffolds to attenuate inflammatory action of astrocytes in vitro and in vivo
dc.typeJournal article
local.bibliographicCitation.issue22
local.bibliographicCitation.lastpage4083
local.bibliographicCitation.startpage4073
local.contributor.affiliationMaclean, Francesca, College of Engineering and Computer Science, ANU
local.contributor.affiliationLau, Chew L, The University of Melbourne
local.contributor.affiliationOzergun, Sam, University of Melbourne
local.contributor.affiliationO'Shea, Ross D., La Trobe University
local.contributor.affiliationCederfur, Cecilia, The University of Melbourne
local.contributor.affiliationWang, J, University of California
local.contributor.affiliationHealy, Kevin E, University of California
local.contributor.affiliationWalker, Frederick R., University of Newcastle
local.contributor.affiliationTomas, Doris, University of Melbourne
local.contributor.affiliationHorne, Malcolm K., University of Melbourne
local.contributor.affiliationBeart, Philip M, The University of Melbourne
local.contributor.affiliationNisbet, David, College of Engineering and Computer Science, ANU
local.contributor.authoremailu5031428@anu.edu.au
local.contributor.authoruidMaclean, Francesca, u4679662
local.contributor.authoruidNisbet, David, u5031428
local.description.notesImported from ARIES
local.identifier.absfor090499 - Chemical Engineering not elsewhere classified
local.identifier.ariespublicationa383154xPUB7405
local.identifier.citationvolume5
local.identifier.doi10.1039/c7tb00651a
local.identifier.scopusID2-s2.0-85021743207
local.identifier.thomsonID000403026400009
local.identifier.uidSubmittedBya383154
local.type.statusPublished Version

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