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Organelle DNA haplotypes reflect crop-use characteristics and geographic origins of Cannabis sativa

Date

2007

Authors

Gilmore, Simon
Peakall, Rodney
Robertson, James

Journal Title

Journal ISSN

Volume Title

Publisher

Elsevier

Abstract

Comparative sequencing of cannabis individuals across 12 chloroplast and mitochondrial DNA loci revealed 7 polymorphic sites, including 5 length variable regions and 2 single nucleotide polymorphisms. Simple PCR assays were developed to assay these polymorphisms, and organelle DNA haplotypes were obtained for 188 cannabis individuals from 76 separate populations, including drug-type, fibre-type and wild populations. The haplotype data were analysed using parsimony, UPGMA and neighbour joining methods. Three haplotype groups were recovered by each analysis method, and these groups are suggestive of the crop-use characteristics and geographical origin of the populations, although not strictly diagnostic. We discuss the relationship between our haplotype data and taxonomic opinions of cannabis, and the implications of organelle DNA haplotyping to forensic investigations of cannabis.

Description

Keywords

Keywords: cannabis; cell DNA; mitochondrial DNA; analytic method; article; cell organelle; chloroplast; comparative study; controlled study; crop; forensic science; gene sequence; geographic distribution; haplotype; human; major clinical study; neighbor joining met Cannabis; Chloroplast; Drugs; Haplotype; Mitochondrial; Organelle

Citation

Source

Forensic Science International

Type

Journal article

Book Title

Entity type

Access Statement

License Rights

DOI

10.1016/j.forsciint.2006.10.025

Restricted until

2037-12-31