The Compact Root Architecture1 Gene Regulates Lignification, Flavonoid Production, and Polar Auxin Transport in Medicago truncatula

Date

2010

Authors

Laffont, Carole
Blanchet, Sandrine
Lapierre, Catherine
Brocard, Lysiane
Ratet, Pascal
Crespi, Martin
Mathesius, Ulrike
Frugier, Florian

Journal Title

Journal ISSN

Volume Title

Publisher

American Society of Plant Biologists

Abstract

The root system architecture is crucial to adapt plant growth to changing soil environmental conditions and consequently to maintain crop yield. In addition to root branching through lateral roots, legumes can develop another organ, the nitrogen-fixing nodule, upon a symbiotic bacterial interaction. A mutant, cra1, showing compact root architecture was identified in the model legume Medicago truncatula. cra1 roots were short and thick due to defects in cell elongation, whereas densities of lateral roots and symbiotic nodules were similar to the wild type. Grafting experiments showed that a lengthened life cycle in cra1 was due to the smaller root system and not to the pleiotropic shoot phenotypes observed in the mutant. Analysis of the cra1 transcriptome at a similar early developmental stage revealed few significant changes, mainly related to cell wall metabolism. The most down-regulated gene in the cra1 mutant encodes a Caffeic Acid O-Methyl Transferase, an enzyme involved in lignin biosynthesis; accordingly, whole lignin content was decreased in cra1 roots. This correlated with differential accumulation of specific flavonoids and decreased polar auxin transport in cra1 mutants. Exogenous application of the isoflavone formononetin to wild-type plants mimicked the cra1 root phenotype, whereas decreasing flavonoid content through silencing chalcone synthases restored the polar auxin transport capacity of the cra1 mutant. The CRA1 gene, therefore, may control legume root growth through the regulation of lignin and flavonoid profiles, leading to changes in polar auxin transport.

Description

Keywords

Keywords: caffeate O methyltransferase; caffeate O-methyltransferase; flavonoid; indoleacetic acid derivative; lignin; methyltransferase; plant RNA; vegetable protein; article; barrel medic; biosynthesis; DNA microarray; gene expression profiling; gene expression r

Citation

Source

Plant Physiology

Type

Journal article

Book Title

Entity type

Access Statement

License Rights

DOI

10.1104/pp.110.156620

Restricted until

2037-12-31