LRGUK-1 is required for basal body and Manchette function during spermatogenesis and male fertility

Date

2015-03-17

Authors

Liu, Yan
DeBoer, Kathleen
de Kretser, David M.
O’Donnell, Liza
O’Connor, Anne E.
Merriner, D. Jo
Okuda, Hidenobu
Whittle, Belinda
Jans, David A.
Efthymiadis, Athina

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Volume Title

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Public Library of Science

Abstract

Male infertility affects at least 5% of reproductive age males. The most common pathology is a complex presentation of decreased sperm output and abnormal sperm shape and motility referred to as oligoasthenoteratospermia (OAT). For the majority of OAT men a precise diagnosis cannot be provided. Here we demonstrate that leucine-rich repeats and guanylate kinase-domain containing isoform 1 (LRGUK-1) is required for multiple aspects of sperm assembly, including acrosome attachment, sperm head shaping and the initiation of the axoneme growth to form the core of the sperm tail. Specifically, LRGUK-1 is required for basal body attachment to the plasma membrane, the appropriate formation of the sub-distal appendages, the extension of axoneme microtubules and for microtubule movement and organisation within the manchette. Manchette dysfunction leads to abnormal sperm head shaping. Several of these functions may be achieved in association with the LRGUK-1 binding partner HOOK2. Collectively, these data establish LRGUK-1 as a major determinant of microtubule structure within the male germ line.

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Source

PLOS Genetics

Type

Journal article

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DOI

10.1371/journal.pgen.1005090

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