Patterned illumination for analyzing neuronal function in 3D

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dc.contributor.authorGo, Mary-Ann
dc.contributor.authorStricker, Christian
dc.contributor.authorRedman, Stephen
dc.contributor.authorBachor, Hans
dc.contributor.authorDaria, Vincent
dc.coverage.spatialBrussels Belgium
dc.date.accessioned2015-12-08T22:16:07Z
dc.date.available2015-12-08T22:16:07Z
dc.date.createdApril 16-19 2012
dc.date.issued2012
dc.date.updated2016-02-24T11:57:59Z
dc.description.abstractWe use patterned 3D multi-spot illumination to perform neuronal multi-site stimulation in rat brain tissue. Using a spatial light modulator, we holograpically project 3D light fields for multi-site two-photon photolysis of caged neurotransmitters to generate synaptic inputs to a neuron. Controlled photostimulation of multiple synapses from various locations in the dendritic tree provides a way to analyze how neurons integrate multiple inputs. Our holographic projection setup is incorporated into a two-photon 3D imaging microscope for visualization and for accurate positioning of specific uncaging sites along the neuron's dendritic tree. We show two-photon images and the neuron's response to holographic photostimulation of synapses along dendrites.
dc.identifier.isbn9780819491190
dc.identifier.urihttp://hdl.handle.net/1885/30528
dc.publisherWiley-VCH Verlag GMBH
dc.relation.ispartofseriesBiophotonics: Photonic Solutions for Better Health Care Conference
dc.sourceProceedings of Biophotonics: Photonic Solutions for Better Health Care Conference
dc.subject3D imaging
dc.subjectHolographic projection
dc.subjectLight fields
dc.subjectMulti-site
dc.subjectMultiple inputs
dc.subjectNeuronal function
dc.subjectPatterned illumination
dc.subjectPhotostimulation
dc.subjectRat brain tissue
dc.subjectSpatial light modulators
dc.subjectSynaptic input
dc.subjectSynaptic integration
dc.subjectTwo photon
dc.subjectForestry
dc.subjectHealth care Holographic projection
dc.subjectSynaptic integration
dc.subjectTwo-photon photolysis
dc.titlePatterned illumination for analyzing neuronal function in 3D
dc.typeConference paper
local.bibliographicCitation.lastpage6
local.bibliographicCitation.startpage842703/1
local.contributor.affiliationGo, Mary-Ann, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationStricker, Christian, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationRedman, Stephen, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationBachor, Hans, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationDaria, Vincent, College of Medicine, Biology and Environment, ANU
local.contributor.authoruidGo, Mary-Ann, u4773901
local.contributor.authoruidStricker, Christian, u4054348
local.contributor.authoruidRedman, Stephen, u8103691
local.contributor.authoruidBachor, Hans, u8203655
local.contributor.authoruidDaria, Vincent, u4492652
local.description.embargo2099-12-31
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.absfor110999 - Neurosciences not elsewhere classified
local.identifier.ariespublicationU9212960xPUB74
local.identifier.doi10.1117/12.923421
local.identifier.scopusID2-s2.0-84861954967
local.identifier.thomsonID000305701200001
local.type.statusPublished Version

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