Migration of Small Ribosomal Subunits on the 5' Untranslated Regions of Capped Messenger RNA

Date

2019-09-10

Authors

Shirokikh, Nikolay
Dutikova, Yulia S.
Staroverova, Maria A.
Hannan, Ross
Preiss, Thomas

Journal Title

Journal ISSN

Volume Title

Publisher

MDPI Publishing

Abstract

Several control mechanisms of eukaryotic gene expression target the initiation step of mRNA translation. The canonical translation initiation pathway begins with cap-dependent attachment of the small ribosomal subunit (SSU) to the messenger ribonucleic acid (mRNA) followed by an energy-dependent, sequential ‘scanning’ of the 50 untranslated regions (UTRs). Scanning through the 50UTR requires the adenosine triphosphate (ATP)-dependent RNA helicase eukaryotic initiation factor (eIF) 4A and its efficiency contributes to the specific rate of protein synthesis. Thus, understanding the molecular details of the scanning mechanism remains a priority task for the field. Here, we studied the effects of inhibiting ATP-dependent translation and eIF4A in cell-free translation and reconstituted initiation reactions programmed with capped mRNAs featuring different 50UTRs. An aptamer that blocks eIF4A in an inactive state away from mRNA inhibited translation of capped mRNA with the moderately structured β-globin sequences in the 50UTR but not that of an mRNA with a poly(A) sequence as the 50UTR. By contrast, the nonhydrolysable ATP analogue β,γ-imidoadenosine 50-triphosphate (AMP-PNP) inhibited translation irrespective of the 50UTR sequence, suggesting that complexes that contain ATP-binding proteins in their ATP-bound form can obstruct and/or actively block progression of ribosome recruitment and/or scanning on mRNA. Further, using primer extension inhibition to locate SSUs on mRNA (‘toeprinting’), we identify an SSU complex which inhibits primer extension approximately eight nucleotides upstream from the usual toeprinting stop generated by SSUs positioned over the start codon. This ‘−8 nt toeprint’ was seen with mRNA 50UTRs of different length, sequence and structure potential. Importantly, the ‘−8 nt toeprint’ was strongly stimulated by the presence of the cap on the mRNA, as well as the presence of eIFs 4F, 4A/4B and ATP, implying active scanning. We assembled cell-free translation reactions with capped mRNA featuring an extended 50UTR and used cycloheximide to arrest elongating ribosomes at the start codon. Impeding scanning through the 50UTR in this system with elevated magnesium and AMP-PNP (similar to the toeprinting conditions), we visualised assemblies consisting of several SSUs together with one full ribosome by electron microscopy, suggesting direct detection of scanning intermediates. Collectively, our data provide additional biochemical, molecular and physical evidence to underpin the scanning model of translation initiation in eukaryotes.

Description

Keywords

eukaryotes, gene expression control, mRNA translation, translation initiation, ribosomal scanning, SSU, 40S ribosomal subunit, cap-dependent initiation, eIF4A, eIF4F, 50UTR, 50 UTR

Citation

Source

International Journal of Molecular Sciences

Type

Journal article

Book Title

Entity type

Access Statement

Open Access

License Rights

Creative Commons Attribution (CC BY) license

DOI

10.3390/ijms20184464

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