Structure of the C123S mutant of dienelactone hydrolase (DLH) bound with the PMS moiety of the protease inhibitor phenylmethylsulfonyl fluoride (PMSF)

Simple item page
dc.contributor.authorRobinson, Anna
dc.contributor.authorEdwards, Karen
dc.contributor.authorCarr, Paul D
dc.contributor.authorBarton, John
dc.contributor.authorEwart, Gary
dc.contributor.authorOllis, David
dc.date.accessioned2015-12-08T22:33:27Z
dc.date.available2015-12-08T22:33:27Z
dc.date.issued2000
dc.date.updated2015-12-08T09:35:58Z
dc.description.abstractThe structure of DLH (C123S) with PMS bound was solved to 2.5 Å resolution (R factor = 15.1%). PMSF in 2-propanol was delivered directly to crystals in drops and unexpectedly caused the crystals to dissolve. New crystals displaying a different morphology
dc.identifier.issn0907-4449
dc.identifier.urihttp://hdl.handle.net/1885/34684
dc.publisherMunksgaard International Publishers
dc.sourceActa Crystallographica Section D: Biological Crystallography
dc.subjectKeywords: benzylsulfonyl fluoride; carboxylesterase; carboxymethylenebutenolidase; proteinase inhibitor; article; chemistry; metabolism; protein conformation; X ray crystallography; Carboxylic Ester Hydrolases; Crystallography, X-Ray; Phenylmethylsulfonyl Fluoride;
dc.titleStructure of the C123S mutant of dienelactone hydrolase (DLH) bound with the PMS moiety of the protease inhibitor phenylmethylsulfonyl fluoride (PMSF)
dc.typeJournal article
local.bibliographicCitation.lastpage1384
local.bibliographicCitation.startpage1376
local.contributor.affiliationRobinson, Anna, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationEdwards, Karen, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationCarr, Paul D, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationBarton, John, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationEwart, Gary, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationOllis, David, College of Physical and Mathematical Sciences, ANU
local.contributor.authoruidRobinson, Anna, u4024790
local.contributor.authoruidEdwards, Karen, u4035073
local.contributor.authoruidCarr, Paul D, u9206448
local.contributor.authoruidBarton, John, u920292
local.contributor.authoruidEwart, Gary, u4033496
local.contributor.authoruidOllis, David, u9200080
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.absfor060107 - Enzymes
local.identifier.ariespublicationMigratedxPub116
local.identifier.citationvolume56
local.identifier.doi10.1107/S0907444900010647
local.identifier.scopusID2-s2.0-0033741880
local.type.statusPublished Version

Downloads

Collections

ANU Research Publications

DSpace software copyright © 2002-2026 LYRASIS