Structure of the C123S mutant of dienelactone hydrolase (DLH) bound with the PMS moiety of the protease inhibitor phenylmethylsulfonyl fluoride (PMSF)

Robinson, Anna; Edwards, Karen; Carr, Paul D; Barton, John; Ewart, Gary; Ollis, David

Structure of the C123S mutant of dienelactone hydrolase (DLH) bound with the PMS moiety of the protease inhibitor phenylmethylsulfonyl fluoride (PMSF)

Date

2000

Authors

Robinson, Anna

Edwards, Karen

Carr, Paul D

Barton, John

Ewart, Gary

Ollis, David

Publisher

Munksgaard International Publishers

Abstract

The structure of DLH (C123S) with PMS bound was solved to 2.5 Å resolution (R factor = 15.1%). PMSF in 2-propanol was delivered directly to crystals in drops and unexpectedly caused the crystals to dissolve. New crystals displaying a different morphology