Cultural advice

The Australian National University acknowledges, celebrates and pays our respects to the Ngunnawal and Ngambri people of the Canberra region and to all First Nations Australians on whose traditional lands we meet and work, and whose cultures are among the oldest continuing cultures in human history.

Aboriginal and Torres Strait Islander peoples are advised that ANU Library collections may include images, names, voices, and other representations of deceased persons.

Material in the collection may contain terms, language or views that reflect the period in which the item was created and may be considered inappropriate today.

An S/MAR-based L1 retrotransposition cassette mediates sustained levels of insertional mutagenesis without suffering from epigenetic silencing of DNA methylation

Date

Authors

Rangasamy, Danny

Publisher

Landes Bioscience

Abstract

L1 is an insertional mutagen that is capable of mediating permanent gene disruption in mammalian genomes. However, currently available L1 retrotransposition vectors exhibit low or unstable transgene expression when expressed in somatic cells and tissues.

Keywords

Keywords: enhanced green fluorescent protein; Epstein Barr virus antigen 1; minicircle DNA; article; cell culture; cell line; cell strain HEK293; controlled study; DNA methylation; epigenetics; gene cassette; gene insertion sequence; gene silencing; gene vector; ge DNA methylation; EBNA1; Episome; Minicircle; Nonviral; Retrotransposition; S/MAR; Somatic mutagenesis; Vector

URI

http://hdl.handle.net/1885/49613

Collections

ANU Research Publications

Source

Epigenetics

Type

Journal article

DOI

10.4161/epi.5.7.12647

Restricted until

2037-12-31

Downloads

File

Description

01_Rangasamy_An_S/MAR-based_L1_2010.pdf (849.38 KB)