Adaptive spatial filtering for off-axis digital holographic microscopy based on region-recognition approach with iterative thresholding

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He, Xuefei
Nguyen, Chuong
Pratap, Mrinalini
Zheng, Yujie
Wang, Yi
Nisbet, David
Rug, Melanie
Maier, Alex
Lee, Woei Ming (Steve)

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SPIE - The International Society for Optical Engineering

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Here we propose a region-recognition approach with iterative thresholding, which is adaptively tailored to extract the appropriate region or shape of spatial frequency. In order to justify the method, we tested it with different samples and imaging conditions (different objectives). We demonstrate that our method provides a useful method for rapid imaging of cellular dynamics in microfluidic and cell cultures.

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Proceedings of SPIE, Conference Volume 10013

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Open Access

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