A second-generation expression system for tyrosine-sulfated proteins and its application in crop protection
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Authors
Schwessinger, Benjamin
Li, Xiang
Ellinghaus, Thomas L.
Chan, Leanne Jade G.
Wei, Tong
Joe, Anna
Thomas, Nicholas
Pruitt, Rory
Adams, Paul D.
Chern, Maw Sheng
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Royal Society of Chemistry
Abstract
Posttranslational modification (PTM) of proteins and peptides is important for diverse biological processes in plants and animals. The paucity of heterologous expression systems for PTMs and the technical challenges associated with chemical synthesis of these modified proteins has limited detailed molecular characterization and therapeutic applications. Here we describe an optimized system for expression of tyrosine-sulfated proteins in Escherichia coli and its application in a bio-based crop protection strategy in rice.
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Integrative Biology
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Open Access