Physical Mapping of Immune Genes in the Tammar Wallaby (Macropus eugenii)
Date
2009
Authors
Sanderson, Claire
Belov, Katherine
Deakin, Janine
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Volume Title
Publisher
S Karger AG
Abstract
The tammar wallaby (Macropus eugenii) is a model marsupial that has recently had its genome sequenced to a depth of 2-fold coverage. Although this is a great resource for comparative genomic studies, information on gene location is essential if this resource is to be used to its full potential. In this study, tammar wallaby bacterial artificial chromosomes (BACs) containing key immune genes were isolated from the tammar wallaby BAC library. BACs containing T cell receptor (TCR) and immunoglobulin (Ig) genes were physically mapped using fluorescence in situ hybridisation (FISH) to tammar wallaby chromosomes. Congruence between the locations of these immune genes in the tammar wallaby genome, with those predicted from chromosome painting data, highlights the conservation of genomic context of these important immune genes in marsupials. The isolation and mapping of these key immune genes in the tammar wallaby will aid in the assembly of the recently sequenced light coverage genome and assignment of sequence to chromosomes.
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Keywords
Keywords: immunoglobulin; T lymphocyte receptor; animal cell; article; bacterial artificial chromosome; chromosome painting; fluorescence in situ hybridization; gene assignment; gene isolation; gene library; gene location; gene mapping; gene sequence; genetic conse Comparative mapping; Immunogenetics; Immunoglobulin; Marsupial; T cell receptor
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Source
Cytogenetic and Genome Research
Type
Journal article
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2037-12-31
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