Atkinesin-13A Modulates Cell-Wall Synthesis and Cell Expansion in Arabidopsis thaliana via the THESEUS1 Pathway
Date
2014
Authors
Fujikura, Ushio
Elsaesser, Lore
Breuninger, Holger
Sanchez-Rodrıguez, Clara
Ivakov, Alexander
Laux, Thomas
Findlay, Kim
Persson, Staffan
Lenhard, Michael
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Public Library of Science
Abstract
Growth of plant organs relies on cell proliferation and expansion. While an increasingly detailed picture about the control of cell proliferation is emerging, our knowledge about the control of cell expansion remains more limited. We demonstrate here that the internal-motor kinesin AtKINESIN-13A (AtKIN13A) limits cell expansion and cell size in Arabidopsis thaliana,
with loss-of-function atkin13a mutants forming larger petals with larger cells. The homolog, AtKINESIN-13B, also affects cell expansion and double mutants display growth, gametophytic and early embryonic defects, indicating a redundant role of the two genes. AtKIN13A is known to depolymerize microtubules and influence Golgi motility and distribution. Consistent with this function, AtKIN13A interacts genetically with ANGUSTIFOLIA, encoding a regulator of Golgi dynamics. Reduced
AtKIN13A activity alters cell wall structure as assessed by Fourier-transformed infrared-spectroscopy and triggers signalling
via the THESEUS1-dependent cell-wall integrity pathway, which in turn promotes the excess cell expansion in the atkin13a mutant. Thus, our results indicate that the intracellular activity of AtKIN13A regulates cell expansion and wall architecture via THESEUS1, providing a compelling case of interplay between cell wall integrity sensing and expansion.
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Keywords: atkinesin 13A; atkinesin 13B; kinesin; unclassified drug; Arabidopsis protein; cell surface receptor; protein kinase; THESEUS1 protein, Arabidopsis; ANGUSTIFOLIA gene; Arabidopsis thaliana; Article; atkin13a gene; biosynthesis; cell damage; cell expansion
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PLoS Genetics
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Journal article
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Open Access
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