Influence of enzymatic maceration on the microstructure and microhardness of compact bone

Date

2010

Authors

Yin, Ling
Venkatesan, Sudharshan
Kalyanasundaram, Shankar
Qin, Qing Hua

Journal Title

Journal ISSN

Volume Title

Publisher

John Wiley & Sons Inc

Abstract

The cleaning of fresh bones to remove their soft tissues while maintaining their structural integrity is a basic and essential part of bone studies. The primary issue is how the cleaning process influences bone microstructures and mechanical properties. We cleaned fresh lamb femurs using enzymatic maceration in comparison with water maceration at room temperature. The microstructures of these compact bones were examined using scanning electron microscopy (SEM) and their porosities were quantified using image processing software. The bone microhardness was measured using a Vickers indentation tester for studying the mechanical properties. The results show that enzymatic maceration of compact bone resulted in a significant microhardness reduction in comparison with water maceration. However, enzymatic maceration did not cause any significant change of porosity in bone structures.

Description

Keywords

Keywords: Bone microstructure; Bone structure; Cleaning process; Compact bone; Image-processing software; Room temperature; SEM; Soft tissue; Vickers indentation; Cleaning; Image processing; Mammals; Mechanical properties; Microhardness; Microstructure; Scanning el

Citation

Source

Journal of Biomedical Materials Research. Part A

Type

Journal article

Book Title

Entity type

Access Statement

License Rights

DOI

10.1088/1748-6041/5/1/015006

Restricted until

2037-12-31