The PWWP domain and the evolution of unique DNA methylation toolkits in Hymenoptera

dc.contributor.authorKucharski, Robert
dc.contributor.authorEllis, Nancy
dc.contributor.authorJurkowski, Tomasz
dc.contributor.authorHurd, Paul J.
dc.contributor.authorMaleszka, Ryszard
dc.date.accessioned2024-08-16T06:00:00Z
dc.date.available2024-08-16T06:00:00Z
dc.date.issued2023
dc.date.updated2024-05-12T08:15:44Z
dc.description.abstractDNMT3 in Hymenoptera has a unique duplication of the essential PWWP domain. Using GST-tagged PWWP fusion proteins and histone arrays we show that these domains have gained new properties and represent the first case of PWWP domains binding to H3K27 chromatin modifications, including H3K27me3, a key modification that is important during development. Phylogenetic analyses of 107 genomes indicate that the duplicated PWWP domains separated into two sister clades, and their distinct binding capacities are supported by 3D modeling. Other features of this unique DNA methylation system include variable copies, losses, and duplications of DNMT1 and DNMT3, and combinatorial generations of DNMT3 isoforms including variants missing the catalytic domain. Some of these losses and duplications of are found only in parasitic wasps. We discuss our findings in the context of the crosstalk between DNA methylation and histone methylation, and the expanded potential of epigenomic modifications in Hymenoptera to drive evolutionary novelties.
dc.format.mimetypeapplication/pdfen_AU
dc.identifier.issn2589-0042
dc.identifier.urihttps://hdl.handle.net/1885/733714818
dc.language.isoen_AUen_AU
dc.provenanceThis is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
dc.publisherCell Press
dc.rights© 2023 The Authors.
dc.rights.licenseCC BY-NC-ND license
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceiScience
dc.subjectEntomology
dc.subjectMolecular biology
dc.subjectEvolutionary biology
dc.titleThe PWWP domain and the evolution of unique DNA methylation toolkits in Hymenoptera
dc.typeJournal article
dcterms.accessRightsOpen Access
local.bibliographicCitation.issue11
local.bibliographicCitation.lastpage13
local.bibliographicCitation.startpage1
local.contributor.affiliationKucharski, Robert, College of Science, ANU
local.contributor.affiliationEllis, Nancy, School of Biological & Behavioural Sciences
local.contributor.affiliationJurkowski, Tomasz, School of Biosciences
local.contributor.affiliationHurd, Paul J., Queen Mary University of London
local.contributor.affiliationMaleszka, Ryszard, College of Science, ANU
local.contributor.authoremailu8709305@anu.edu.au
local.contributor.authoruidKucharski, Robert, u9612185
local.contributor.authoruidMaleszka, Ryszard, u8709305
local.description.notesImported from ARIES
local.identifier.absfor310504 - Epigenetics (incl. genome methylation and epigenomics)
local.identifier.ariespublicationa383154xPUB44346
local.identifier.citationvolume26
local.identifier.doi10.1016/j.isci.2023.108193
local.identifier.scopusID2-s2.0-85174719484
local.identifier.uidSubmittedBya383154
local.publisher.urlhttps://www.sciencedirect.com/
local.type.statusPublished Version
publicationvolume.volumeNumber26

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