Proofreading exonuclease on a tether: the complex between the E. coli DNA polymerase III subunits α, ε, θ and β reveals a highly flexible arrangement of the proofreading domain

Ozawa, Kiyoshi; Horan, Nicholas P; Robinson, Andrew; Yagi, Hiromasa; Hill, Flynn R; Jergic, Slobodan; Xu, Zhi-Qiang; Loscha, Karin; Li, Nan; Tehei, Moeava; Oakley, Aaron; Dixon, Nicholas Edward; Otting, Gottfried; Huber, Thomas

Proofreading exonuclease on a tether: the complex between the E. coli DNA polymerase III subunits α, ε, θ and β reveals a highly flexible arrangement of the proofreading domain

Date

2013

Authors

Ozawa, Kiyoshi

Horan, Nicholas P

Robinson, Andrew

Yagi, Hiromasa

Hill, Flynn R

Jergic, Slobodan

Xu, Zhi-Qiang

Loscha, Karin

Li, Nan

Tehei, Moeava

Publisher

Oxford University Press

Abstract

A complex of the three (αεθ) core subunits and the β2 sliding clamp is responsible for DNA synthesis by Pol III, the Escherichia coli chromosomal DNA replicase. The 1.7 Å crystal structure of a complex between the PHP domain of α (polymerase) and th