A pair of allelic WRKY genes play opposite roles in rice-bacteria interactions

Date

2009

Authors

Tao, Zeng
Liu, Hongbo
Qiu, Deyun
Zhou, Yan
Li, Xianghua
xu, Caiguo
Wang, Shiping

Journal Title

Journal ISSN

Volume Title

Publisher

American Society of Plant Biologists

Abstract

Although allelic diversity of genes has been reported to play important roles in different physiological processes, information on allelic diversity of defense-responsive genes in host-pathogen interactions is limited. Here, we report that a pair of allelic genes, OsWRKY45-1 and OsWRKY45-2, which encode proteins with a 10-amino acid difference, play opposite roles in rice (Oryza sativa) resistance against bacterial pathogens. Bacterial blight caused by Xanthomonas oryzae pv oryzae (Xoo), bacterial streak caused by Xanthomonas oryzae pv oryzicola (Xoc), and fungal blast caused by Magnaporthe grisea are devastating diseases of rice worldwide. OsWRKY45-1-overexpressing plants showed increased susceptibility and OsWRKY45-1-knockout plants showed enhanced resistance to Xoo and Xoc. In contrast, OsWRKY45-2-overexpressing plants showed enhanced resistance and OsWRKY45-2-suppressing plants showed increased susceptibility to Xoo and Xoc. Interestingly, both OsWRKY45-1- and OsWRKY45-2-overexpressing plants showed enhanced resistance to M. grisea. OsWRKY45-1-regulated Xoo resistance was accompanied by increased accumulation of salicylic acid and jasmonic acid and induced expression of a subset of defense-responsive genes, while OsWRKY45-2-regulated Xoo resistance was accompanied by increased accumulation of jasmonic acid but not salicylic acid and induced expression of another subset of defense-responsive genes. These results suggest that both OsWRKY45-1 and OsWRKY45-2 are positive regulators in rice resistance against M. grisea, but the former is a negative regulator and the latter is a positive regulator in rice resistance against Xoo and Xoc. The opposite roles of the two allelic genes in rice-Xoo interaction appear to be due to their mediation of different defense signaling pathways.

Description

Keywords

Keywords: cyclopentane derivative; jasmonic acid; oxylipin; salicylic acid; transcription factor; vegetable protein; allele; amino acid sequence; article; chemistry; gene expression regulation; genetics; host pathogen interaction; immunology; innate immunity; Magna

Citation

Source

Plant Physiology

Type

Journal article

Book Title

Entity type

Access Statement

License Rights

DOI

10.1104/pp.109.145623

Restricted until

2037-12-31