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Reconstitution of a nanomachine driving the assembly of proteins into bacterial outer membranes

Shen, Hsin-Hui; Leyton, Denisse; Shiota, Takuya; Belousoff, Matthew J; Noinaj, Nicholas; Lu, Jingxiong; Holt, Stephen A.; Tan, Kershing; Selkrig, Joel; Webb, Chaille T.; Buchanan, Susan K.; Martin, Lisandra; Lithgow, Trevor


In biological membranes, various protein secretion devices function as nanomachines, and measuring the internal movements of their component parts is a major technological challenge. The translocation and assembly module (TAM) is a nanomachine required for virulence of bacterial pathogens. We have reconstituted a membrane containing the TAM onto a gold surface for characterization by quartz crystal microbalance with dissipation (QCM-D) and magnetic contrast neutron reflectrometry (MCNR). The...[Show more]

CollectionsANU Research Publications
Date published: 2014
Type: Journal article
Source: Nature Communications
DOI: 10.1038/ncomms6078


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