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Identification of I-7 expands the repertoire of genes for resistance to Fusarium wilt in tomato to three resistance gene classes

Gonzalez Cendales, Yvonne; Catanzariti, Ann-Maree; Baker, Barbara; McGrath, D. J.; Jones, David

Description

The tomato I-3 and I-7 genes confer resistance to Fusarium oxysporum f. sp. lycopersici (Fol) race 3 and were introgressed into the cultivated tomato, Solanum lycopersicum, from the wild relative Solanum pennellii. I-3 has been identified previously on chromosome 7 and encodes an S-receptor-like kinase, but little is known about I-7. Molecular markers have been developed for the marker-assisted breeding of I-3, but none are available for I-7. We used an RNA-seq and single nucleotide...[Show more]

dc.contributor.authorGonzalez Cendales, Yvonne
dc.contributor.authorCatanzariti, Ann-Maree
dc.contributor.authorBaker, Barbara
dc.contributor.authorMcGrath, D. J.
dc.contributor.authorJones, David
dc.date.accessioned2016-02-24T22:41:07Z
dc.identifier.issn1464-6722
dc.identifier.urihttp://hdl.handle.net/1885/98574
dc.description.abstractThe tomato I-3 and I-7 genes confer resistance to Fusarium oxysporum f. sp. lycopersici (Fol) race 3 and were introgressed into the cultivated tomato, Solanum lycopersicum, from the wild relative Solanum pennellii. I-3 has been identified previously on chromosome 7 and encodes an S-receptor-like kinase, but little is known about I-7. Molecular markers have been developed for the marker-assisted breeding of I-3, but none are available for I-7. We used an RNA-seq and single nucleotide polymorphism (SNP) analysis approach to map I-7 to a small introgression of S.pennelliiDNA (c. 210kb) on chromosome 8, and identified I-7 as a gene encoding a leucine-rich repeat receptor-like protein (LRR-RLP), thereby expanding the repertoire of resistance protein classes conferring resistance to Fol. Using an eds1 mutant of tomato, we showed that I-7, like many other LRR-RLPs conferring pathogen resistance in tomato, is EDS1 (Enhanced Disease Susceptibility 1) dependent. Using transgenic tomato plants carrying only the I-7 gene for Fol resistance, we found that I-7 also confers resistance to Fol races 1 and 2. Given that Fol race 1 carries Avr1, resistance to Fol race 1 indicates that I-7-mediated resistance, unlike I-2- or I-3-mediated resistance, is not suppressed by Avr1. This suggests that Avr1 is not a general suppressor of Fol resistance in tomato, leading us to hypothesize that Avr1 may be acting against an EDS1-independent pathway for resistance activation. The identification of I-7 has allowed us to develop molecular markers for marker-assisted breeding of both genes currently known to confer Fol race 3 resistance (I-3 and I-7). Given that I-7-mediated resistance is not suppressed by Avr1, I-7 may be a useful addition to I-3 in the tomato breeder's toolbox.
dc.publisherBlackwell Publishing Ltd
dc.sourceMolecular Plant Pathology
dc.titleIdentification of I-7 expands the repertoire of genes for resistance to Fusarium wilt in tomato to three resistance gene classes
dc.typeJournal article
local.description.notesImported from ARIES
dc.date.issued2015
local.identifier.absfor060704 - Plant Pathology
local.identifier.absfor070603 - Horticultural Crop Protection (Pests, Diseases and Weeds)
local.identifier.ariespublicationU3488905xPUB6076
local.type.statusPublished Version
local.contributor.affiliationGonzalez Cendales, Yvonne, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationCatanzariti, Ann-Maree, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationBaker, Barbara, University of California
local.contributor.affiliationMcGrath, D J, QLD Department of Primary Industries and Fisheries
local.contributor.affiliationJones, David, College of Medicine, Biology and Environment, ANU
local.description.embargo2037-12-31
local.identifier.doi10.1111/mpp.12294
local.identifier.absseo970106 - Expanding Knowledge in the Biological Sciences
local.identifier.absseo820299 - Horticultural Crops not elsewhere classified
dc.date.updated2016-02-24T10:09:40Z
local.identifier.scopusID2-s2.0-84941883225
CollectionsANU Research Publications

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