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Gd3+ Spin Labeling for Measuring Distances in Biomacromolecules: Why and How?

Feintuch, Akiva; Goldfarb, Daniella; Otting, Gottfried

Description

Applications of distance measurements by pulse dipolar electron-paramagnetic resonance (PD-EPR) spectroscopy to structural biology are based on introducing spin labels (SLs) at well-defined locations in the biomacromolecule. The most commonly used SLs are nitroxyl radicals, but recently SLs based on high-spin Gd3 + (S = 7/2) complexes have been shown to be an attractive alternative for PD-EPR, particularly double electron-electron resonance (DEER), at spectrometer frequencies higher than 30...[Show more]

CollectionsANU Research Publications
Date published: 2015
Type: Journal article
URI: http://hdl.handle.net/1885/98549
Source: Methods in Enzymology
DOI: 10.1016/bs.mie.2015.07.006

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