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Small-angle neutron scattering study of the ultrastructure of chloroplast thylakoid membranes - Periodicity and structural flexibility of the stroma lamellae

Timmins, P.; Posselt, D; Kirkensgaard, Jacob; Nagy, Gergely; Holm, J.K.; Aagaard, Thomas H.; Retfalvi, Eszter; Rosta, Laszlo; Kovacs, Laszlo; Garab, Gyozo

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The multilamellar organization of freshly isolated spinach and pea chloroplast thylakoid membranes was studied using small-angle neutron scattering. A broad peak at ∼ 0.02 Å- 1 is ascribed to diffraction from domains of ordered, unappressed stroma lamellae, revealing a repeat distance of 294 Å ± 7 Å in spinach and 345 Å ± 11 Å in pea. The peak position and hence the repeat distance of stroma lamellae is strongly dependent on the osmolarity and the ionic strength of the suspension medium, as...[Show more]

dc.contributor.authorTimmins, P.
dc.contributor.authorPosselt, D
dc.contributor.authorKirkensgaard, Jacob
dc.contributor.authorNagy, Gergely
dc.contributor.authorHolm, J.K.
dc.contributor.authorAagaard, Thomas H.
dc.contributor.authorRetfalvi, Eszter
dc.contributor.authorRosta, Laszlo
dc.contributor.authorKovacs, Laszlo
dc.contributor.authorGarab, Gyozo
dc.date.accessioned2016-02-24T22:40:49Z
dc.identifier.issn0005-2728
dc.identifier.urihttp://hdl.handle.net/1885/98455
dc.description.abstractThe multilamellar organization of freshly isolated spinach and pea chloroplast thylakoid membranes was studied using small-angle neutron scattering. A broad peak at ∼ 0.02 Å- 1 is ascribed to diffraction from domains of ordered, unappressed stroma lamellae, revealing a repeat distance of 294 Å ± 7 Å in spinach and 345 Å ± 11 Å in pea. The peak position and hence the repeat distance of stroma lamellae is strongly dependent on the osmolarity and the ionic strength of the suspension medium, as demonstrated by varying the sorbitol and the Mg++-concentration in the sample. For pea thylakoid membranes, we show that the repeat distance decreases when illuminating the sample with white light, in accordance with our earlier results on spinach, also regarding the observation that addition of an uncoupler prohibits the light-induced structural changes, a strong indication that these changes are driven by the transmembrane proton gradient. We show that the magnitude of the shrinkage is strongly dependent on light intensity and that the repeat distance characteristic of the dark state after illumination is different from the initial dark state. Prolonged strong illumination leads to irreversible changes and swelling as reflected in increased repeat distances. The observed reorganizations are discussed within the frames of the current structural models of the granum-stroma thylakoid membrane assembly and the regulatory mechanisms in response to variations in the environmental conditions in vivo. This article is part of a Special Issue entitled: Photosynthesis Research for Sustainability: from Natural to Artificial.
dc.publisherElsevier
dc.sourceBiochimica et Biophysica Acta: Bioenergetics
dc.subjectKeywords: proton; sorbitol; chemical structure; chloroplast; conference paper; illumination; neutron scattering; observation; osmolarity; osmotic pressure; photosynthesis; priority journal; thylakoid membrane; Light; Magnesium; Neutrons; Osmotic Pressure; Peas; Sca Granum; Light-induced reorganization; Repeat distance; Small-angle neutron scattering (SANS); Stroma thylakoid membrane; Transmembrane proton gradient
dc.titleSmall-angle neutron scattering study of the ultrastructure of chloroplast thylakoid membranes - Periodicity and structural flexibility of the stroma lamellae
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume1817
dc.date.issued2012
local.identifier.absfor010599 - Mathematical Physics not elsewhere classified
local.identifier.ariespublicationU3488905xPUB4157
local.type.statusPublished Version
local.contributor.affiliationTimmins, P., Institut Laue-Langevin
local.contributor.affiliationPosselt, D, University of Roskilde
local.contributor.affiliationKirkensgaard, Jacob, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationNagy, Gergely, Institut Laue-Langevin
local.contributor.affiliationHolm, J.K., University of Roskilde
local.contributor.affiliationAagaard, Thomas H., Roskilde University
local.contributor.affiliationRetfalvi, Eszter, Hungarian Academy of Sciences
local.contributor.affiliationRosta, Laszlo, Hungarian Academy of Sciences
local.contributor.affiliationKovacs, Laszlo, Hungarian Academy of Sciences
local.contributor.affiliationGarab, Gyozo, Hungarian Academy of Sciences
local.description.embargo2037-12-31
local.bibliographicCitation.issue8
local.bibliographicCitation.startpage1220
local.bibliographicCitation.lastpage1228
local.identifier.doi10.1016/j.bbabio.2012.01.012
dc.date.updated2016-02-24T10:06:05Z
local.identifier.scopusID2-s2.0-84862224970
CollectionsANU Research Publications

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