Enzyme studies in the tricarboxylic acid cycle : the purification and properties of aconitase
Morrison, John Francis
The purpose of this investigation was to obtain a highly purified preparation of aconitase and to study properties of the purified enzyme. The thesis describes: (I) The method of purification (II) The activation of aconitase by Fe2+ and reducing agents (III) The effect of pH on the enzyme activity (IV) A study of the kinetics of the enzyme (V) The effect of Fluorocitric acid on the enzyme activity (VI) The effect of other inhibitors on the enzyme activity. The result may be briefly summarised...[Show more]
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