670 nm light mitigates oxygen-induced degeneration in C57BL/6J mouse retina
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Albarracin, Rizalyn; Natoli, Riccardo; Rutar, Matthew; Valter, Krisztina; Provis, Jan
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BACKGROUND Irradiation with light wavelengths from the far red (FR) to the near infrared (NIR) spectrum (600 nm -1000 nm) has been shown to have beneficial effects in several disease models. In this study, we aim to examine whether 670 nm red light pretreatment can provide protection against hyperoxia-induced damage in the C57BL/6J mouse retina. Adult mice (90-110 days) were pretreated with 9 J/cm2 of 670 nm light once daily for 5 consecutive days prior to being placed in hyperoxic environment...[Show more]
dc.contributor.author | Albarracin, Rizalyn | |
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dc.contributor.author | Natoli, Riccardo | |
dc.contributor.author | Rutar, Matthew | |
dc.contributor.author | Valter, Krisztina | |
dc.contributor.author | Provis, Jan | |
dc.date.accessioned | 2016-01-24T22:26:16Z | |
dc.date.available | 2016-01-24T22:26:16Z | |
dc.identifier.issn | 1471-2202 | |
dc.identifier.uri | http://hdl.handle.net/1885/95644 | |
dc.description.abstract | BACKGROUND Irradiation with light wavelengths from the far red (FR) to the near infrared (NIR) spectrum (600 nm -1000 nm) has been shown to have beneficial effects in several disease models. In this study, we aim to examine whether 670 nm red light pretreatment can provide protection against hyperoxia-induced damage in the C57BL/6J mouse retina. Adult mice (90-110 days) were pretreated with 9 J/cm2 of 670 nm light once daily for 5 consecutive days prior to being placed in hyperoxic environment (75% oxygen). Control groups were exposed to hyperoxia, but received no 670 nm light pretreatment. Retinas were collected after 0, 3, 7, 10 or 14 days of hyperoxia exposure (n = 12/group) and prepared either for histological analysis, or RNA extraction and quantitative polymerase chain reaction (qPCR). Photoreceptor damage and loss were quantified by counting photoreceptors undergoing cell death and measuring photoreceptor layer thickness. Localization of acrolein, and cytochrome c oxidase subunit Va (Cox Va) were identified through immunohistochemistry. Expression of heme oxygenase-1 (Hmox-1), complement component 3 (C3) and fibroblast growth factor 2 (Fgf-2) genes were quantified using qPCR. RESULTS The hyperoxia-induced photoreceptor loss was accompanied by reduction of metabolic marker, Cox Va, and increased expression of oxidative stress indicator, acrolein and Hmox-1. Pretreatment with 670 nm red light reduced expression of markers of oxidative stress and C3, and slowed, but did not prevent, photoreceptor loss over the time course of hyperoxia exposure. CONCLUSION The damaging effects of hyperoxia on photoreceptors were ameliorated following pretreatment with 670 nm light in hyperoxic mouse retinas. These results suggest that pretreatment with 670 nm light may provide stability to photoreceptors in conditions of oxidative stress. | |
dc.description.sponsorship | This work was supported by the Australian Research Council Centre of Excellence in Vision Science. | |
dc.publisher | BioMed Central | |
dc.rights | © Albarracin et al.; licensee BioMed Central Ltd. 2013 This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | |
dc.source | BMC Neuroscience | |
dc.subject | animals | |
dc.subject | cell survival | |
dc.subject | female | |
dc.subject | hyperoxia | |
dc.subject | immunohistochemistry | |
dc.subject | in situ nick-end labeling | |
dc.subject | male | |
dc.subject | mice | |
dc.subject | mice, inbred c57bl | |
dc.subject | microscopy, confocal | |
dc.subject | oxidative stress | |
dc.subject | photoreceptor cells, vertebrate | |
dc.subject | real-time polymerase chain reaction | |
dc.subject | retina | |
dc.subject | retinal degeneration | |
dc.subject | infrared rays | |
dc.title | 670 nm light mitigates oxygen-induced degeneration in C57BL/6J mouse retina | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.identifier.citationvolume | 14 | |
dc.date.issued | 2013-10-17 | |
local.identifier.absfor | 110900 | |
local.identifier.ariespublication | f5625xPUB6580 | |
local.publisher.url | http://www.biomedcentral.com/ | |
local.type.status | Published Version | |
local.contributor.affiliation | Albarracin, Rizalyn, College of Medicine, Biology and Environment, CMBE Research School of Biology, Division of Plant Sciences, The Australian National University | |
local.contributor.affiliation | Natoli, Riccardo, College of Medicine, Biology and Environment, CMBE John Curtin School of Medical Research, Eccles Institute of Neuroscience, The Australian National University | |
local.contributor.affiliation | Rutar, Matthew, College of Medicine, Biology and Environment, CMBE John Curtin School of Medical Research, Eccles Institute of Neuroscience, The Australian National University | |
local.contributor.affiliation | Valter (Valter-Kocsi), Krisztina, College of Medicine, Biology and Environment, CMBE John Curtin School of Medical Research, Eccles Institute of Neuroscience, The Australian National University | |
local.contributor.affiliation | Provis, Jan, College of Medicine, Biology and Environment, CMBE John Curtin School of Medical Research, Eccles Institute of Neuroscience, The Australian National University | |
local.identifier.essn | 1471-2202 | |
local.bibliographicCitation.issue | 1 | |
local.bibliographicCitation.startpage | 125 | |
local.identifier.doi | 10.1186/1471-2202-14-125 | |
dc.date.updated | 2016-02-24T09:30:24Z | |
local.identifier.scopusID | 2-s2.0-84885531551 | |
local.identifier.thomsonID | 000326575300002 | |
Collections | ANU Research Publications |
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