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Selective and flexible depletion of problematic sequences from RNA-seq libraries at the cDNA stage

Archer, Stuart K; Shirokikh, Nikolay E; Preiss, Thomas

Description

BACKGROUND A major hurdle to transcriptome profiling by deep-sequencing technologies is that abundant transcripts, such as rRNAs, can overwhelm the libraries, severely reducing transcriptome-wide coverage. Methods for depletion of such unwanted sequences typically require treatment of RNA samples prior to library preparation, are costly and not suited to unusual species and applications. Here we describe Probe-Directed Degradation (PDD), an approach that employs hybridisation to DNA...[Show more]

CollectionsANU Research Publications
Date published: 2014-05-26
Type: Journal article
URI: http://hdl.handle.net/1885/95565
Source: BMC Genomics
DOI: 10.1186/1471-2164-15-401

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