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Measurement of Epstein-Barr virus DNA load using a novel quantification standard containing two EBV DNA targets and SYBR Green I dye

Lay, Meav-Lang J; Lucas, Robyn; Ratnamohan, Mala; Taylor, Janette; Ponsonby, Anne-Louise; Dwyer, Dominic E; Ausimmune Investigator Group (AIG)

Description

BACKGROUND Reactivation of Epstein-Barr virus (EBV) infection may cause serious, life-threatening complications in immunocompromised individuals. EBV DNA is often detected in EBV-associated disease states, with viral load believed to be a reflection of virus activity. Two separate real-time quantitative polymerase chain reaction (QPCR) assays using SYBR Green I dye and a single quantification standard containing two EBV genes, Epstein-Barr nuclear antigen-1 (EBNA-1) and BamHI fragment H...[Show more]

CollectionsANU Research Publications
Date published: 2010-09-22
Type: Journal article
URI: http://hdl.handle.net/1885/95324
Source: Virology Journal
DOI: 10.1186/1743-422X-7-252

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