Cho, Vicky; Mei, Yan; Sanny, Arleen; Chan, Stephanie; Enders, Anselm; Bertram, Edward M; Tan, Andy; Goodnow, Christopher C; Andrews, T
BACKGROUND Retention of a subset of introns in spliced polyadenylated mRNA is emerging as a frequent, unexplained finding from RNA deep sequencing in mammalian cells. RESULTS Here we analyze intron retention in T lymphocytes by deep sequencing polyadenylated RNA. We show a developmentally regulated RNA-binding protein, hnRNPLL, induces retention of specific introns by sequencing RNA from T cells with an inactivating Hnrpll mutation and from B lymphocytes that physiologically downregulate Hnrpll...[Show more]
Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.