Skip navigation
Skip navigation

The genome of the Tiger Milk mushroom, Lignosus rhinocerotis, provides insights into the genetic basis of its medicinal properties

Yap, Hui-Yeng Y; Chooi, Yit-Heng; Firdaus-Raih, Mohd; Fung, Shin-Yee; Ng, Szu-Ting; Tan, Chon-Seng; Tan, Nget-Hong

Description

BACKGROUND The sclerotium of Lignosus rhinocerotis (Cooke) Ryvarden or Tiger milk mushroom (Polyporales, Basidiomycota) is a valuable folk medicine for indigenous peoples in Southeast Asia. Despite the increasing interest in this ethnobotanical mushroom, very little is known about the molecular and genetic basis of its medicinal and nutraceutical properties. RESULTS The de novo assembled 34.3 Mb L. rhinocerotis genome encodes 10,742 putative genes with 84.30% of them having detectable sequence...[Show more]

dc.contributor.authorYap, Hui-Yeng Y
dc.contributor.authorChooi, Yit-Heng
dc.contributor.authorFirdaus-Raih, Mohd
dc.contributor.authorFung, Shin-Yee
dc.contributor.authorNg, Szu-Ting
dc.contributor.authorTan, Chon-Seng
dc.contributor.authorTan, Nget-Hong
dc.date.accessioned2015-12-21T04:44:50Z
dc.date.available2015-12-21T04:44:50Z
dc.identifier.issn1471-2164
dc.identifier.urihttp://hdl.handle.net/1885/95147
dc.description.abstractBACKGROUND The sclerotium of Lignosus rhinocerotis (Cooke) Ryvarden or Tiger milk mushroom (Polyporales, Basidiomycota) is a valuable folk medicine for indigenous peoples in Southeast Asia. Despite the increasing interest in this ethnobotanical mushroom, very little is known about the molecular and genetic basis of its medicinal and nutraceutical properties. RESULTS The de novo assembled 34.3 Mb L. rhinocerotis genome encodes 10,742 putative genes with 84.30% of them having detectable sequence similarities to others available in public databases. Phylogenetic analysis revealed a close evolutionary relationship of L. rhinocerotis to Ganoderma lucidum, Dichomitus squalens, and Trametes versicolor in the core polyporoid clade. The L. rhinocerotis genome encodes a repertoire of enzymes engaged in carbohydrate and glycoconjugate metabolism, along with cytochrome P450s, putative bioactive proteins (lectins and fungal immunomodulatory proteins) and laccases. Other genes annotated include those encoding key enzymes for secondary metabolite biosynthesis, including those from polyketide, nonribosomal peptide, and triterpenoid pathways. Among them, the L. rhinocerotis genome is particularly enriched with sesquiterpenoid biosynthesis genes. CONCLUSIONS The genome content of L. rhinocerotis provides insights into the genetic basis of its reported medicinal properties as well as serving as a platform to further characterize putative bioactive proteins and secondary metabolite pathway enzymes and as a reference for comparative genomics of polyporoid fungi.
dc.description.sponsorshipThis research is supported by High Impact Research Grant UM.C/625/1/HIR/ MoE/E20040-20001 from the University of Malaya/Ministry of Education, Malaysia. H-YYY is supported by the postgraduate research grant (PPP) PV024/ 2012A from University of Malaya, Malaysia. Y-HC is a recipient of Australian Research Council Discovery Early Career Researcher Award (ARC DECRA).
dc.publisherBioMed Central
dc.rights© 2014 Yap et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated
dc.sourceBMC Genomics
dc.subjectfungal proteins
dc.subjectgenes, fungal
dc.subjectphylogeny
dc.subjectpolyporales
dc.subjectpolysaccharides
dc.subjectgenomics
dc.subjectmedicine, east asian traditional
dc.titleThe genome of the Tiger Milk mushroom, Lignosus rhinocerotis, provides insights into the genetic basis of its medicinal properties
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume15
dc.date.issued2014-07-29
local.identifier.absfor030401
local.identifier.absfor060102
local.identifier.ariespublicationU3488905xPUB5095
local.type.statusPublished Version
local.contributor.affiliationYap, Hui-Yeng Y, University of Malaya, Malaysia
local.contributor.affiliationChooi, Yit-Heng, College of Medicine, Biology and Environment, CMBE Research School of Biology, Division of Plant Sciences, The Australian National University
local.contributor.affiliationFirdaus-Raih, Mohd, Universiti Kebangsaan Malaysia, Malaysia
local.contributor.affiliationFung, Shin-Yee, University of Malaya, Malaysia
local.contributor.affiliationNg, Szu-Ting, Ligno Biotech Sdn. Bhd, Malaysia
local.contributor.affiliationTan, Chon-Seng, Malaysian Agricultural Research and Development Institute (MARDI), Malaysia
local.contributor.affiliationTan, Nget-Hong, University of Malaya, Malaysia
local.identifier.essn1471-2164
local.bibliographicCitation.issue1
local.bibliographicCitation.startpage635
local.identifier.doi10.1186/1471-2164-15-635
local.identifier.absseo860803
dc.date.updated2016-02-24T10:06:51Z
local.identifier.scopusID2-s2.0-84904783654
local.identifier.thomsonID000339972700001
dcterms.accessRightsOpen Access
CollectionsANU Research Publications

Download

File Description SizeFormat Image
01_Yap_The_genome_of_the_Tiger_Milk_2014.pdfPublished Version1.55 MBAdobe PDFThumbnail


Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.

Updated:  19 May 2020/ Responsible Officer:  University Librarian/ Page Contact:  Library Systems & Web Coordinator