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Cholinergic synaptic transmission in insect mushroom bodies in vitro

Oleskevich, Sharon

Description

The mushroom body of the bee brain is an important site for learning and memory. Here we investigate synaptic transmission in the mushroom body using extracellular recording techniques in a whole bee brain in vitro preparation. The postsynaptic response showed attenuation by cadmium and paired-pulse facilitation, similar to in vivo findings. This confirms the viability of the in vitro preparation and supports the isolated whole bee brain as a useful model of the in vivo preparation. Bath...[Show more]

dc.contributor.authorOleskevich, Sharon
dc.date.accessioned2015-12-13T23:40:21Z
dc.date.available2015-12-13T23:40:21Z
dc.identifier.issn0022-3077
dc.identifier.urihttp://hdl.handle.net/1885/94427
dc.description.abstractThe mushroom body of the bee brain is an important site for learning and memory. Here we investigate synaptic transmission in the mushroom body using extracellular recording techniques in a whole bee brain in vitro preparation. The postsynaptic response showed attenuation by cadmium and paired-pulse facilitation, similar to in vivo findings. This confirms the viability of the in vitro preparation and supports the isolated whole bee brain as a useful model of the in vivo preparation. Bath application of the acetylcholine receptor antagonists, D-tubocurarine and α-bungarotoxin attenuated the postsynaptic response by 61 and 62% of control, respectively. The glutamate receptor antagonists, (+)-2-amino-5-phosphonopentanoic acid and 6-cyano-7- nitroquinoxaline-2,3-dione, had no effect. The invertebrate monoamine and neuromodulator, octopamine, transiently increased the postsynaptic response by 130% of control. These results suggest that synaptic transmission of the olfactory input pathway in the mushroom body is 1) mediated primarily by acetylcholine and 2) modulated by octopamine.
dc.publisherAmerican Physiological Society
dc.sourceJournal of Neurophysiology
dc.subjectKeywords: 2 amino 5 phosphonovaleric acid; 6 cyano 7 nitro 2,3 quinoxalinedione; alpha bungarotoxin; cholinergic receptor; cholinergic receptor blocking agent; glutamate receptor; glutamate receptor antagonist; octopamine; tubocurarine chloride; article; cholinergi
dc.titleCholinergic synaptic transmission in insect mushroom bodies in vitro
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume82
dc.date.issued1999
local.identifier.absfor060105 - Cell Neurochemistry
local.identifier.ariespublicationMigratedxPub24014
local.type.statusPublished Version
local.contributor.affiliationOleskevich, Sharon, College of Medicine, Biology and Environment, ANU
local.bibliographicCitation.startpage1091
local.bibliographicCitation.lastpage1096
dc.date.updated2015-12-12T09:28:52Z
local.identifier.scopusID2-s2.0-0032800409
CollectionsANU Research Publications

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