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The mitochondrial genome can be altered or lost without lethal effect in the petie-negative yeast Debarymyces (Schwanniomyces) occidentalis

Fernet, C; Clark-Walker, George; Claisse, M

Description

The nature of mutations affecting several cytochrome-deficient mutants of Debaryomyces (Schwanniomyces) occidentalis has been characterized. The DR12 mutant, which is deficient in cytochrome b, and the B10Mn mutant, which is deficient in cytochromes b and a, a3, are deleted in the mitochondrial CYB and COX1 genes respectively. The B10 strain, which is partially deficient in cytochrome b, has no detectable change in its mitochondrial DNA and possibly carries nuclear lesion(s). These three...[Show more]

dc.contributor.authorFernet, C
dc.contributor.authorClark-Walker, George
dc.contributor.authorClaisse, M
dc.date.accessioned2015-12-13T23:40:11Z
dc.identifier.issn0172-8083
dc.identifier.urihttp://hdl.handle.net/1885/94342
dc.description.abstractThe nature of mutations affecting several cytochrome-deficient mutants of Debaryomyces (Schwanniomyces) occidentalis has been characterized. The DR12 mutant, which is deficient in cytochrome b, and the B10Mn mutant, which is deficient in cytochromes b and a, a3, are deleted in the mitochondrial CYB and COX1 genes respectively. The B10 strain, which is partially deficient in cytochrome b, has no detectable change in its mitochondrial DNA and possibly carries nuclear lesion(s). These three mutants, unlike the rho- and rhoo "petite" mutants of Saccharomyces cerevisiae, can still grow on non-fermentable substrates, due to the development of a salicylhydroxamic acid (SHAM)-sensitive alternative pathway linked to phosphorylation at site 1. A gly- mutant lacking mtDNA and respiratory capacity has been isolated. For the first time, it is demonstrated that mtDNA can be altered or even lost without lethal consequence in D. occidentalis, although this yeast was classified as a petite-negative species.
dc.publisherSpringer
dc.sourceCurrent Genetics
dc.subjectKeywords: chloramphenicol; cytochrome a; cytochrome b; erythromycin; mitochondrial DNA; mitochondrial protein; salicylhydroxamic acid; absorption spectroscopy; classification; controlled study; Debaryomyces; Debaryomyces occidentalis; DNA determination; drug effect Debaryomyces (Schwanniomyces) occidentalis; Mitochondrial DNA; Petite-negative
dc.titleThe mitochondrial genome can be altered or lost without lethal effect in the petie-negative yeast Debarymyces (Schwanniomyces) occidentalis
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume42
dc.date.issued2002
local.identifier.absfor060407 - Genome Structure and Regulation
local.identifier.ariespublicationMigratedxPub23909
local.type.statusPublished Version
local.contributor.affiliationFernet, C, CNRS
local.contributor.affiliationClark-Walker, George, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationClaisse, M, CNRS
local.description.embargo2037-12-31
local.bibliographicCitation.startpage94
local.bibliographicCitation.lastpage102
local.identifier.doi10.1007/s00294-002-0337-4
dc.date.updated2015-12-12T09:28:10Z
local.identifier.scopusID2-s2.0-0036872170
CollectionsANU Research Publications

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