Molecular and global time-resolved analysis of a psbS genedosage effect on pH and xanthophyll cycle-dependent nonphotochemical quenching in photosystem 11
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Li, Xiao-Ping; Gilmore, Adam; Niyogi, Krishna
Description
Photosynthetic light harvesting in plants is regulated by a pH- and xanthophyll-dependent nonphotochemical quenching process (qE) that dissipates excess absorbed light energy and requires the psbS gene product. An Arabidopsis thaliana mutant, npq4-1, lacks qE because of a deletion of the psbS gene, yet it exhibits a semidominant phenotype. Here it is shown that the semidominance is due to a psbS gene dosage effect. Diploid Arabidopsis plants containing two psbS gene copies (wild-type), one psbS...[Show more]
dc.contributor.author | Li, Xiao-Ping | |
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dc.contributor.author | Gilmore, Adam | |
dc.contributor.author | Niyogi, Krishna | |
dc.date.accessioned | 2015-12-13T23:40:06Z | |
dc.identifier.issn | 0021-9258 | |
dc.identifier.uri | http://hdl.handle.net/1885/94305 | |
dc.description.abstract | Photosynthetic light harvesting in plants is regulated by a pH- and xanthophyll-dependent nonphotochemical quenching process (qE) that dissipates excess absorbed light energy and requires the psbS gene product. An Arabidopsis thaliana mutant, npq4-1, lacks qE because of a deletion of the psbS gene, yet it exhibits a semidominant phenotype. Here it is shown that the semidominance is due to a psbS gene dosage effect. Diploid Arabidopsis plants containing two psbS gene copies (wild-type), one psbS gene (npq4-1/NPQ4 heterozygote), and no psbS gene (npq4-1/npq4-1 homozygote) were compared. Heterozygous plants had 56% of the wild-type psbS mRNA level, 58% of the wild-type PsbS protein level, and 60% of the wild-type level of qE. Global analysis of the chlorophyll a fluorescence lifetime distributions revealed three components in wild-type and heterozygous plants, but only a single long lifetime component in npq4-1. The short lifetime distribution associated with qE was inhibited by more than 40% in heterozygous plants compared with the wild type. Thus, the extent of qE measured as either the fractional intensities of the PSII chlorophyll a fluorescence lifetime distributions or steady state intensities was stoichiometrically related to the amount of PsbS protein. | |
dc.publisher | American Society for Biochemistry and Molecular Biology Inc | |
dc.source | Journal of Biological Chemistry | |
dc.subject | Keywords: Chlorophyll; Fluorescence; Genes; Photosynthesis; Plants (botany); Quenching; Gene dosages; Biochemistry; chlorophyll a; gene product; messenger RNA; protein psbs; unclassified drug; xanthophyll; Arabidopsis; article; fluorescence; gene dosage; gene expre | |
dc.title | Molecular and global time-resolved analysis of a psbS genedosage effect on pH and xanthophyll cycle-dependent nonphotochemical quenching in photosystem 11 | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.description.refereed | Yes | |
local.identifier.citationvolume | 277 | |
dc.date.issued | 2002 | |
local.identifier.absfor | 070303 - Crop and Pasture Biochemistry and Physiology | |
local.identifier.ariespublication | MigratedxPub23867 | |
local.type.status | Published Version | |
local.contributor.affiliation | Li, Xiao-Ping, University of California | |
local.contributor.affiliation | Gilmore, Adam, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Niyogi, Krishna, University of California | |
local.description.embargo | 2037-12-31 | |
local.bibliographicCitation.startpage | 33590 | |
local.bibliographicCitation.lastpage | 33597 | |
local.identifier.doi | 10.1074/jbc.M204797200 | |
dc.date.updated | 2015-12-12T09:27:48Z | |
local.identifier.scopusID | 2-s2.0-0037072764 | |
Collections | ANU Research Publications |
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