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Reassessment of major products of N 2 fixation by bacteroids from soybean root nodules

Li, Youzhong; Parsons, R; Day, David Alexander; Bergersen, F

Description

NH3/NH4+ was the principal product from soybean bacteroids, prepared by various procedures, when assayed in solution in a flow chamber under N2 fixation conditions. In addition, small quantities of alanine were produced (reaching 20% of NH3/NH4+ under some conditions). Some 15N was assimilated by bacteroids purified from soybean root nodules on Percoll density gradients and shaken with 15N2 and 0.008 atm O2. Under these conditions, 15NH4+ accounted for 93% of the 15N fixed into the soluble...[Show more]

CollectionsANU Research Publications
Date published: 2002
Type: Journal article
URI: http://hdl.handle.net/1885/94202
Source: Microbiology (UK)

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