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Integron-sequestered dihydrofolate reductase: a recently redeployed enzyme

Alonso, Hernan; Gready, Jill

Description

The introduction and wide use of antibacterial drugs has resulted in the emergence of resistant organisms. DfrB dihydrofolate reductase (DHFR) is a bacterial enzyme that is uniquely associated with mobile gene cassettes within integrons, and confers resistance to the drug trimethoprim. This enzyme has intrigued microbiologists since it was discovered more than thirty years ago because of its simple structure, enzymatic inefficiency and its virtual insensitivity to trimethoprim. Here, for the...[Show more]

dc.contributor.authorAlonso, Hernan
dc.contributor.authorGready, Jill
dc.date.accessioned2015-12-13T23:35:43Z
dc.identifier.issn0966-842X
dc.identifier.urihttp://hdl.handle.net/1885/94038
dc.description.abstractThe introduction and wide use of antibacterial drugs has resulted in the emergence of resistant organisms. DfrB dihydrofolate reductase (DHFR) is a bacterial enzyme that is uniquely associated with mobile gene cassettes within integrons, and confers resistance to the drug trimethoprim. This enzyme has intrigued microbiologists since it was discovered more than thirty years ago because of its simple structure, enzymatic inefficiency and its virtual insensitivity to trimethoprim. Here, for the first time, a comprehensive discussion of genetic, evolutionary, structural and functional studies of this enzyme is presented together. This information supports the ideas that DfrB DHFR is a poorly adapted catalyst and has recently been recruited to perform a novel enzymatic activity in response to selective pressure.
dc.publisherElsevier
dc.sourceTrends in Microbiology
dc.subjectKeywords: bacterial enzyme; dihydrofolate reductase; trimethoprim; Aeromonas hydrophila; Aeromonas salmonicida; antibiotic resistance; bacterial gene; bacterial genetics; Bordetella bronchiseptica; enzyme activity; enzyme analysis; enzyme structure; Escherichia col
dc.titleIntegron-sequestered dihydrofolate reductase: a recently redeployed enzyme
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume14
dc.date.issued2006
local.identifier.absfor060199 - Biochemistry and Cell Biology not elsewhere classified
local.identifier.absfor060107 - Enzymes
local.identifier.absfor060499 - Genetics not elsewhere classified
local.identifier.ariespublicationMigratedxPub25495
local.type.statusPublished Version
local.contributor.affiliationAlonso, Hernan, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationGready, Jill, College of Medicine, Biology and Environment, ANU
local.description.embargo2037-12-31
local.bibliographicCitation.issue5
local.bibliographicCitation.startpage236
local.bibliographicCitation.lastpage242
local.identifier.doi10.1016/j.tim.2006.03.003
dc.date.updated2015-12-12T09:40:58Z
local.identifier.scopusID2-s2.0-33745879777
CollectionsANU Research Publications

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