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Murine histidine-rich glycoprotein: Cloning, characterisation and cellular origin

Hulett, Mark; Parish, Christopher

Description

Histidine-rich glycoprotein (HRG) is a plasma protein of vertebrates that has been implicated in the regulation of several important biological functions, including the immune response and blood clotting. In the present study, we have isolated and determined the sequence of the cDNAs for both mouse and rat HRG. The deduced amino acid sequences of mouse and rat HRG are 525 and 510 amino acids, respectively, and they show the same three-domain structure that has been predicted for human HRG, with...[Show more]

dc.contributor.authorHulett, Mark
dc.contributor.authorParish, Christopher
dc.date.accessioned2015-12-13T23:35:36Z
dc.identifier.issn0818-9641
dc.identifier.urihttp://hdl.handle.net/1885/93982
dc.description.abstractHistidine-rich glycoprotein (HRG) is a plasma protein of vertebrates that has been implicated in the regulation of several important biological functions, including the immune response and blood clotting. In the present study, we have isolated and determined the sequence of the cDNAs for both mouse and rat HRG. The deduced amino acid sequences of mouse and rat HRG are 525 and 510 amino acids, respectively, and they show the same three-domain structure that has been predicted for human HRG, with which they share high amino acid identity. Northern blot analysis indicates that the mouse HRG mRNA is 1.7 kb and is localized specifically to the liver. It has been suggested, somewhat controversially, that some immune cells, such as monocytes and megakaryocytes, also synthesize HRG. Reverse transcriptase-polymerase chain reaction analysis has failed to show any HRG mRNA in immune tissues of the mouse, including the spleen, thymus, lymph node, bone marrow and peripheral blood leucocytes. These data suggest that HRG expression by immune cells is due to the acquisition of plasma HRG derived from the liver. Finally, genomic Southern blot analysis of the mouse HRG gene suggests that it is a single copy gene.
dc.publisherBlackwell Publishing Ltd
dc.sourceImmunology and Cell Biology
dc.subjectKeywords: glycoprotein; histidine; amino acid sequence; article; molecular cloning; nonhuman; Northern blotting; nucleotide sequence; protein analysis; protein isolation; protein localization; reverse transcription polymerase chain reaction; Animals; Base Sequence; cDNA cloning; Histidine-proline-rich glycoprotein; Histidine-rich glycoprotein
dc.titleMurine histidine-rich glycoprotein: Cloning, characterisation and cellular origin
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume78
dc.date.issued2000
local.identifier.absfor110106 - Medical Biochemistry: Proteins and Peptides (incl. Medical Proteomics)
local.identifier.ariespublicationMigratedxPub25428
local.type.statusPublished Version
local.contributor.affiliationHulett, Mark, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationParish, Christopher, College of Medicine, Biology and Environment, ANU
local.description.embargo2037-12-31
local.bibliographicCitation.issue3
local.bibliographicCitation.startpage280
local.bibliographicCitation.lastpage287
local.identifier.doi10.1046/j.1440-1711.2000.00940.x
dc.date.updated2015-12-12T09:40:37Z
local.identifier.scopusID2-s2.0-0034088242
CollectionsANU Research Publications

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