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Nuclear-accumulation kinetics of p9CksHs1 and p9CksHs2 in live plant cells correlate with immunochemical characteristics

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Williams, Elizabeth; Hepler, P; Carrello, A; John, Peter Crook

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The two human homologues of the fission yeast cell cycle protein p13(suc1) displayed structural characteristics consistent with their existing in solution as differently folded monomers despite 81% identity with respect to their primary structures and both being capable of fulfilling the functions of their homologues in fission and budding yeasts. Carboxyfluorescein-labelled p(9CksHs1) and p(9CksH2) retained their native structures. When microinjected into live stamen hair cells of Tradescantia...[Show more]

dc.contributor.authorWilliams, Elizabeth
dc.contributor.authorHepler, P
dc.contributor.authorCarrello, A
dc.contributor.authorJohn, Peter Crook
dc.date.accessioned2015-12-13T23:35:02Z
dc.identifier.issn0033-183X
dc.identifier.urihttp://hdl.handle.net/1885/93723
dc.description.abstractThe two human homologues of the fission yeast cell cycle protein p13(suc1) displayed structural characteristics consistent with their existing in solution as differently folded monomers despite 81% identity with respect to their primary structures and both being capable of fulfilling the functions of their homologues in fission and budding yeasts. Carboxyfluorescein-labelled p(9CksHs1) and p(9CksH2) retained their native structures. When microinjected into live stamen hair cells of Tradescantia virginiana, the labelled proteins accumulated in the nuclei of the cells. Markedly different nuclear-accumulation kinetics indicated that the human proteins interact differently with other cellular constituents, which supports the proposition that they may have different roles in cellular regulation.
dc.publisherSpringer
dc.sourceProtoplasma
dc.subjectKeywords: chemical analysis; immunoanalysis; Tradescantia virginiana Cell division; Nucleus; P(9CksHs1); P(9CksHs2); P13(suc1); Tradescantia virginiana
dc.titleNuclear-accumulation kinetics of p9CksHs1 and p9CksHs2 in live plant cells correlate with immunochemical characteristics
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume207
dc.date.issued1999
local.identifier.absfor060106 - Cellular Interactions (incl. Adhesion, Matrix, Cell Wall)
local.identifier.ariespublicationMigratedxPub25117
local.type.statusPublished Version
local.contributor.affiliationWilliams, Elizabeth, Cornell University
local.contributor.affiliationHepler, P, University of Massachusetts
local.contributor.affiliationCarrello, A, University of Western Australia
local.contributor.affiliationJohn, Peter Crook, College of Medicine, Biology and Environment, ANU
local.description.embargo2037-12-31
local.bibliographicCitation.startpage98
local.bibliographicCitation.lastpage105
dc.date.updated2015-12-12T09:38:25Z
local.identifier.scopusID2-s2.0-0032988342
CollectionsANU Research Publications

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