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Virulence of mousepox is independent of serpin-mediated control of cellular cytotoxicity

Wallich, Reinhard; Simon, Markus M; Mullbacher, Arno

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We have investigated whether the differential virulence seen of two Ectromelia (Ect) strains, EctMoscow and EctHampstead egg, is due to mutation or differential regulation of their serpins (SPI). Poxvirus encoded serine proteinase inhibitors (serpins) have been shown to interfere with cytolytic activity of leukocytes and can also determine virulence. We show that the deduced amino acid sequences of SPI-1, 2, and 3 are identical for the highly virulent EctMoscow and the low virulent EctHampstead...[Show more]

dc.contributor.authorWallich, Reinhard
dc.contributor.authorSimon, Markus M
dc.contributor.authorMullbacher, Arno
dc.date.accessioned2015-12-13T23:27:02Z
dc.identifier.issn0882-8245
dc.identifier.urihttp://hdl.handle.net/1885/93128
dc.description.abstractWe have investigated whether the differential virulence seen of two Ectromelia (Ect) strains, EctMoscow and EctHampstead egg, is due to mutation or differential regulation of their serpins (SPI). Poxvirus encoded serine proteinase inhibitors (serpins) have been shown to interfere with cytolytic activity of leukocytes and can also determine virulence. We show that the deduced amino acid sequences of SPI-1, 2, and 3 are identical for the highly virulent EctMoscow and the low virulent EctHampstead strains and that the two viruses express similar potential to inhibit T-cell cytotoxicity, in particular, Fas-mediated target cell lysis, by allorective effectors. Virus titres in wild type B6 mice were effectively controlled very early after inoculation with EctHampstead as compared with EctMoscow, but lack of perforin renders B6 mice similarly susceptible to both virus strains. The data demonstrate that in Ect infection the perforin-mediated cytolytic pathway is not the primary target of serpins and suggest that the apparent attenuation of EctHampstead seen in B6 mice is due to control elements distinct from SPI-1, 2, and 3.
dc.publisherMary Ann Liebert Inc.
dc.sourceViral Immunology
dc.subjectKeywords: perforin; serine proteinase inhibitor; transcription factor Sp1; transcription factor Sp3; amino acid sequence; animal cell; animal experiment; animal model; animal tissue; article; controlled study; cytolysis; cytotoxicity; Ectromelia virus; female; gene
dc.titleVirulence of mousepox is independent of serpin-mediated control of cellular cytotoxicity
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume14
dc.date.issued2001
local.identifier.absfor110804 - Medical Virology
local.identifier.ariespublicationMigratedxPub26467
local.type.statusPublished Version
local.contributor.affiliationWallich, Reinhard, Max Planck Institute of Immunobiology
local.contributor.affiliationSimon, Markus M, Max Planck Institute of Immunobiology
local.contributor.affiliationMullbacher, Arno, College of Medicine, Biology and Environment, ANU
local.description.embargo2037-12-31
local.bibliographicCitation.issue1
local.bibliographicCitation.startpage71
local.bibliographicCitation.lastpage81
dc.date.updated2015-12-12T09:48:46Z
local.identifier.scopusID2-s2.0-0035106094
CollectionsANU Research Publications

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