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Cell surface expression of the 300 kDa mannose-6-phosphate receptor by activated T lymphocytes

Hindmarsh, Elizabeth; Staykova, Maria; Willenborg, D; Parish, Christopher

Description

Phosphosugars, such as mannose-6-phosphate (M6P), have been shown previously to display anti-inflammatory properties, notably inhibition of experimental autoimmune encephalomyelitis (EAE) and adjuvant-induced arthritis in rats. It has been proposed that M6P exerts its anti-inflammatory effect by displacing lysosomal enzymes, which are involved in T-cell extravasation into inflammatory sites, from the 300 kDa mannose-6-phosphate receptor (MPR-300) on the surface of T cells. If this model is...[Show more]

dc.contributor.authorHindmarsh, Elizabeth
dc.contributor.authorStaykova, Maria
dc.contributor.authorWillenborg, D
dc.contributor.authorParish, Christopher
dc.date.accessioned2015-12-13T23:27:00Z
dc.identifier.issn0818-9641
dc.identifier.urihttp://hdl.handle.net/1885/93112
dc.description.abstractPhosphosugars, such as mannose-6-phosphate (M6P), have been shown previously to display anti-inflammatory properties, notably inhibition of experimental autoimmune encephalomyelitis (EAE) and adjuvant-induced arthritis in rats. It has been proposed that M6P exerts its anti-inflammatory effect by displacing lysosomal enzymes, which are involved in T-cell extravasation into inflammatory sites, from the 300 kDa mannose-6-phosphate receptor (MPR-300) on the surface of T cells. If this model is correct MPR-300 should be selectively expressed on the surface of activated T cells, as T cell entry into the central nervous system in EAE depends on the T cells being in an activated state. Thus, the present study examines whether cell surface expression of MPR-300 by T lymphocytes correlates with their state of activation and whether T cells in inflammatory sites express the receptor. Flow cytometric studies showed MPR-300 to be absent from the surface of unstimulated rat T cells isolated from peripheral blood and lymphoid tissues, and T cells resident within the peritoneal cavity. In contrast, MPR-300 was expressed on activated T cells derived from an inflammatory peritoneal exudate. In vitro studies demonstrated transient expression of MPR-300 on the surface of splenic T cells following stimulation with Con A. MPR-300 was also induced on T-cell lines by antigen stimulation. These data demonstrate that T cells in inflammatory sites express MPR-300 on their surface and activation of T lymphocytes induces cell surface expression of MPR-300. Such findings are consistent with the hypothesis that cell surface MPR-300 is required for the entry of T cells into inflammatory sites.
dc.publisherBlackwell Publishing Ltd
dc.sourceImmunology and Cell Biology
dc.subjectKeywords: CD4 antigen; CD45 antigen; CD8 antigen; concanavalin A; interleukin 2 receptor; lymphocyte function associated antigen 1; membrane antigen; monoclonal antibody; somatomedin B receptor; thioglycolic acid; allergic encephalomyelitis; animal model; animal ti Inflammation; Lymphocyte extravasation; Mannose-6-phosphate receptor; T-lymphocyte activation
dc.titleCell surface expression of the 300 kDa mannose-6-phosphate receptor by activated T lymphocytes
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume79
dc.date.issued2001
local.identifier.absfor110703 - Autoimmunity
local.identifier.ariespublicationMigratedxPub26450
local.type.statusPublished Version
local.contributor.affiliationHindmarsh, Elizabeth, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationStaykova, Maria, Canberra Hospital
local.contributor.affiliationWillenborg, D, Canberra Hospital
local.contributor.affiliationParish, Christopher, College of Medicine, Biology and Environment, ANU
local.description.embargo2037-12-31
local.bibliographicCitation.issue5
local.bibliographicCitation.startpage436
local.bibliographicCitation.lastpage443
local.identifier.doi10.1046/j.1440-1711.2001.01026.x
dc.date.updated2015-12-12T09:48:40Z
local.identifier.scopusID2-s2.0-0035570431
CollectionsANU Research Publications

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