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Characteristics of Irreversible ATP Activation Suggest that Native Skeletal Ryanodine Receptors Can Be Phosphorylated via an Endogenous CaMKII

Dulhunty, Angela; Laver, Derek Rowland; Curtis, Suzanne; Pace, Suzy M; Haarmann, Claudia; Gallant, Esther

Description

Phosphorylation of skeletal muscle ryanodine receptor (RyR) calcium release channels by endogenous kinases incorporated into lipid bilayers with native sarcoplasmic reticulum vesicles was investigated during exposure to 2 mM cytoplasmic ATP. Activation of RyRs after 1-min exposure to ATP was reversible upon ATP washout. In contrast, activation after 5 to 8 min was largely irreversible: the small fall in activity with washout was significantly less than that after brief ATP exposure. The...[Show more]

dc.contributor.authorDulhunty, Angela
dc.contributor.authorLaver, Derek Rowland
dc.contributor.authorCurtis, Suzanne
dc.contributor.authorPace, Suzy M
dc.contributor.authorHaarmann, Claudia
dc.contributor.authorGallant, Esther
dc.date.accessioned2015-12-13T23:26:34Z
dc.identifier.issn0006-3495
dc.identifier.urihttp://hdl.handle.net/1885/92893
dc.description.abstractPhosphorylation of skeletal muscle ryanodine receptor (RyR) calcium release channels by endogenous kinases incorporated into lipid bilayers with native sarcoplasmic reticulum vesicles was investigated during exposure to 2 mM cytoplasmic ATP. Activation of RyRs after 1-min exposure to ATP was reversible upon ATP washout. In contrast, activation after 5 to 8 min was largely irreversible: the small fall in activity with washout was significantly less than that after brief ATP exposure. The irreversible activation was reduced by acid phosphatase and was not seen after exposure to nonhydrolyzable ATP analogs. The data suggested that the channel complex was phosphorylated after addition of ATP and that phosphorylation reduced the RyR's sensitivity to ATP, adenosine, and Ca2+. The endogenous kinase was likely to be a calcium calmodulin kinase II (CaMKII) because the CaMKII inhibitor KN-93 and an inhibitory peptide for CaMKII prevented the phosphorylation-induced irreversible activation. In contrast, phosphorylation effects remained unchanged with inhibitory peptides for protein kinase C and A. The presence of CaMKIIβ in the SR vesicles was confirmed by immunoblotting. The results suggest that CaMKII is anchored to skeletal muscle RyRs and that phosphorylation by this kinase alters the enhancement of channel activity by ATP and Ca2+.
dc.publisherBiophysical Society
dc.sourceBiophysical Journal
dc.subjectKeywords: acid phosphatase; adenosine; adenosine triphosphate; adenosine triphosphate derivative; calcium ion; cyclic AMP dependent protein kinase; n [2 [[n [3 (4 chlorophenyl) 2 propenyl] n methylamino]methyl]phenyl] n (2 hydroxyethyl) 4 methoxybenzenesulfonamide;
dc.titleCharacteristics of Irreversible ATP Activation Suggest that Native Skeletal Ryanodine Receptors Can Be Phosphorylated via an Endogenous CaMKII
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume81
dc.date.issued2001
local.identifier.absfor060601 - Animal Physiology - Biophysics
local.identifier.ariespublicationMigratedxPub26144
local.type.statusPublished Version
local.contributor.affiliationDulhunty, Angela, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationLaver, Derek Rowland, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationCurtis, Suzanne, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationPace, Suzy M, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationHaarmann, Claudia, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationGallant, Esther, University of Minnesota
local.description.embargo2037-12-31
local.bibliographicCitation.startpage3240
local.bibliographicCitation.lastpage3252
dc.date.updated2015-12-12T09:47:07Z
local.identifier.scopusID2-s2.0-0035200868
CollectionsANU Research Publications

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