A NF-kB/Sp1 Region Is Essential for Chromatin Remodeling and Correct Transcription of a Human Granulocyte-Macrophage Colony-Stimulating Factor Transgene
The GM-CSF gene is expressed following activation of T cells. The proximal promoter and an upstream enhancer have previously been characterized using transfection and reporter assays in T cell lines in culture. A 10.5-kb transgene containing the entire human GM-CSF gene has also been shown to display inducible, position-independent, copy number-dependent transcription in mouse splenocytes. To determine the role of individual promoter elements in transgene function, mutations were introduced...[Show more]
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|Source:||Journal of Immunology|
|01_Cakouros_A_NF-kB/Sp1_Region_Is_2001.pdf||1.69 MB||Adobe PDF||Request a copy|
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