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Chloroplast Cu/Zn-SOD is a highly sensitive site in cucumber leaves chilled in the light

Choi, Sun Mi; Jeong, Suk Weon; Jeong, Won; Kwon, Seung Ho; Chow, Wah S (Fred); Park, Youn-Il

Description

Light-chilling stress, the combination of low-light illumination and low temperature, preferentially inactivated photosystem I (PSI) of cucumber (Cucumis sativus L.) leaves, resulting in the photoinhibition of photosynthesis. The extent of PSI photoinhibition, determined in vivo by monitoring absorption changes around 810 nm (induced by far-red light), was closely correlated with the redox state of the PSII electron acceptor QA, measured as the chlorophyll fluorescence parameter, 1-qP, where qP...[Show more]

dc.contributor.authorChoi, Sun Mi
dc.contributor.authorJeong, Suk Weon
dc.contributor.authorJeong, Won
dc.contributor.authorKwon, Seung Ho
dc.contributor.authorChow, Wah S (Fred)
dc.contributor.authorPark, Youn-Il
dc.date.accessioned2015-12-13T23:26:25Z
dc.identifier.issn0032-0935
dc.identifier.urihttp://hdl.handle.net/1885/92820
dc.description.abstractLight-chilling stress, the combination of low-light illumination and low temperature, preferentially inactivated photosystem I (PSI) of cucumber (Cucumis sativus L.) leaves, resulting in the photoinhibition of photosynthesis. The extent of PSI photoinhibition, determined in vivo by monitoring absorption changes around 810 nm (induced by far-red light), was closely correlated with the redox state of the PSII electron acceptor QA, measured as the chlorophyll fluorescence parameter, 1-qP, where qP is a photochemical quenching coefficient. In contrast, the decrease in the far-red-induced leaf absorptance signal was not well correlated with the limited fragmentation of the PsaA/B gene products in the PSI reaction center after the light-chilling stress. Amongst various enzymes involved in the photooxidative damage such as superoxide dismutase (SOD), ascorbate peroxidase, and NAD(P)H dehydrogenase, only SOD was inhibited by light-chilling treatment. Further, an approximately 3-fold increase in the leaf content of H2O2, a potent inhibitor of Cu/Zn-SOD, was observed after light-chilling stress. From these results, we suggest that Cu/Zn-SOD is the primary target of the light-chilling stress, followed by subsequent inactivation of PSI by reactive oxygen species.
dc.publisherSpringer
dc.sourcePlanta
dc.subjectKeywords: Chlorophyll; Enzymes; Light absorption; Lighting; Photosynthesis; Quenching; Photosystems; Plants (botany); Cucumis; Cucumis sativus; ascorbate peroxidase; chlorophyll; hydrogen peroxide; oxygen; peroxidase; reactive oxygen metabolite; reduced nicotinamid Cu/Zn-superoxide dismutase; Cucumis; Light-chilling; Photoinhibition; Photosystem I
dc.titleChloroplast Cu/Zn-SOD is a highly sensitive site in cucumber leaves chilled in the light
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume216
dc.date.issued2002
local.identifier.absfor030499 - Medicinal and Biomolecular Chemistry not elsewhere classified
local.identifier.ariespublicationMigratedxPub26052
local.type.statusPublished Version
local.contributor.affiliationChoi, Sun Mi, Chungnam National University
local.contributor.affiliationJeong, Suk Weon, Chungnam National University
local.contributor.affiliationJeong, Won, Korea Research Institute for Bioscience and Biotechnology
local.contributor.affiliationKwon, Seung Ho, University of New South Wales
local.contributor.affiliationChow, Wah S (Fred), College of Medicine, Biology and Environment, ANU
local.contributor.affiliationPark, Youn-Il, Chungnam National University
local.description.embargo2037-12-31
local.bibliographicCitation.startpage315
local.bibliographicCitation.lastpage324
local.identifier.doi10.1007/s00425-002-0852-z
dc.date.updated2015-12-12T09:46:31Z
local.identifier.scopusID2-s2.0-0036886255
CollectionsANU Research Publications

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